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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Nucleotide sequence of the regulatory region of the uvrD gene of Escherichia coli.

We have sequenced the control region of the Escherichia coli uvrD gene and demonstrated the presence of a nucleotide sequence which is a perfect match for the consensus LexA protein binding site [Little and Mount, Cell 29 (1982) 11-22]. Upstream of this presumed LexA binding site is a promoter sequence, uvrD P1 which would be under LexA control while farther downstream is another possible promoter, uvrD P2, which would be independent of LexA control. Downstream of the LexA binding site is a potential transcription terminator in the form of a stem-loop structure followed by a series of T residues. On the basis of this sequence analysis, expression of the uvrD gene would be expected to increase after DNA damage or replication inhibition as part of the SOS response, as is reported in the preceding paper [Arthur and Eastlake, Gene 25 (1983) 309-316].[1]


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