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Gene Review

uvrD  -  DNA-dependent ATPase I and helicase II

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3808, JW3786, dar-2, dda, mutU, ...
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Disease relevance of uvrD


High impact information on uvrD

  • The Escherichia coli uvrD gene product, helicase II, is required for both methyl-directed mismatch and uvrABC excision repair and is believed to function by unwinding duplex DNA [6].
  • A 2.9-kilobase (kb) Pvu II DNA fragment that contains the uvrD gene of Escherichia coli K-12 has been cloned in both low-copy and multiple-copy plasmid vehicles [7].
  • Instead, norfloxacin causes DNA damage, as evidenced by the induction of the SOS pathway for DNA repair; the increase in susceptibility to the drug by mutations in genes for DNA repair pathways including recA, recB, and uvrD; and the efficient detergent-induced linearization of plasmid DNA in drug-treated cells [8].
  • These results are relevant to the suspected role of the dda protein in phage T4 DNA replication, as well as to its possible role in phage genetic recombination [9].
  • Here, we report the cloning and sequencing of two additional P. aeruginosa mismatch repair genes and the characterization, by complementation of deficient strains, of these two putative P. aeruginosa mismatch repair genes (mutL and uvrD) [10].

Chemical compound and disease context of uvrD


Biological context of uvrD

  • These results indicate that the uvrD gene is induced during the SOS response, and that the expression of the gene may also be regulated by transcription attenuation [1].
  • The 1190 base pairs of DNA examined include the likely coding sequence for the first 258 amino acids of the uvrD protein [1].
  • The transcription promoter for the uvrD gene was identified upstream of the protein coding region [1].
  • Surprisingly, the uvrD deletion completely suppressed the phenotype of a ruvB mutation in a recBCsbcB(C) background [13].
  • In addition, the constitutive expression of uvrD was reduced in strains containing either the recA56 mutation or a multi-copy plasmid carrying lexA+ [14].

Anatomical context of uvrD

  • Using this assay, DNA damages generated in calf thymus, phi X174 RF, and f1 single-stranded DNA, X-irradiated in phosphate buffer, were easily detectable at 10 rad (0.1 Gy) [15].

Associations of uvrD with chemical compounds

  • The interactions of the inhibitory effects of the uvrD, exaA, and recB mutations and chloramphenicol treatment were determined by examining the survival and repair characteristics of the several multiple mutants [16].
  • These results indicate that the uvrD mutation inhibits certain rec+lex+-dependent repair processes, including the growth-medium-dependent (Type III) repair of X-ray-induced DNA single-strand breaks, but does not inhibit other rec+lex+-dependent processes that are sensitive to 2,4-dinitrophenol and chloramphenicol [17].
  • Alkaline sucrose gradient sedimentation studies indicated that the uvrD strain is deficient in the growth-medium-dependent (Type III) repair of DNA single-strand breaks [17].
  • These data suggest that the ultraviolet sensitivity conferred by the uvrD mutation arises from inefficient removal of pyrimidine dimers or from failure to close incision breaks [18].
  • We sequenced >250 mutations leading to Rif(r) in D. radiodurans derived spontaneously in wild-type and uvrD (mismatch-repair-deficient) backgrounds and after treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and 5-azacytidine (5AZ) [19].

Physical interactions of uvrD

  • The addition of the uvrD protein to the incised DNA-uvrABC complex also results in turnover of only the uvrC protein [20].

Other interactions of uvrD


Analytical, diagnostic and therapeutic context of uvrD


  1. Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation. Easton, A.M., Kushner, S.R. Nucleic Acids Res. (1983) [Pubmed]
  2. Overexpression, purification, DNA binding, and dimerization of the Escherichia coli uvrD gene product (helicase II). Runyon, G.T., Wong, I., Lohman, T.M. Biochemistry (1993) [Pubmed]
  3. The Salmonella typhimurium LT2 uvrD gene is regulated by the lexA gene product. Pang, P.P., Walker, G.C. J. Bacteriol. (1983) [Pubmed]
  4. Cloning and characterization of the uvrD gene from an extremely thermophilic bacterium, Thermus thermophilus HB8. Hiramatsu, Y., Kato, R., Kawaguchi, S., Kuramitsu, S. Gene (1997) [Pubmed]
  5. Cloning and characterization of the Haemophilus influenzae mutB gene. Stuy, J.H., Walter, R.B. J. Bacteriol. (1993) [Pubmed]
  6. Escherichia coli helicase II (UvrD) protein initiates DNA unwinding at nicks and blunt ends. Runyon, G.T., Bear, D.G., Lohman, T.M. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  7. DNA repair in Escherichia coli: identification of the uvrD gene product. Maples, V.F., Kushner, S.R. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  8. The mechanism of inhibition of topoisomerase IV by quinolone antibacterials. Khodursky, A.B., Cozzarelli, N.R. J. Biol. Chem. (1998) [Pubmed]
  9. Purification and characterization of the bacteriophage T4 dda protein. A DNA helicase that associates with the viral helix-destabilizing protein. Jongeneel, C.V., Formosa, T., Alberts, B.M. J. Biol. Chem. (1984) [Pubmed]
  10. The mismatch repair system (mutS, mutL and uvrD genes) in Pseudomonas aeruginosa: molecular characterization of naturally occurring mutants. Oliver, A., Baquero, F., Blázquez, J. Mol. Microbiol. (2002) [Pubmed]
  11. Escherichia coli uvrD mutants with thermosensitive DNA-dependent adenosine triphosphatase I (helicase II). Richet, E., Nishimura, Y., Hirota, Y., Kohiyama, M. Mol. Gen. Genet. (1983) [Pubmed]
  12. Role of the uvrE gene product and of inducible O6-methylguanine removal in the induction of mutations by N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli. Sklar, R., Strauss, B. J. Mol. Biol. (1980) [Pubmed]
  13. Genetic analysis of delta helD and delta uvrD mutations in combination with other genes in the RecF recombination pathway in Escherichia coli: suppression of a ruvB mutation by a uvrD deletion. Mendonca, V.M., Matson, S.W. Genetics (1995) [Pubmed]
  14. Transcriptional control of the uvrD gene of Escherichia coli. Arthur, H.M., Eastlake, P.B. Gene (1983) [Pubmed]
  15. A novel, sensitive, and specific assay for abasic sites, the most commonly produced DNA lesion. Kubo, K., Ide, H., Wallace, S.S., Kow, Y.W. Biochemistry (1992) [Pubmed]
  16. Genetic control of multiple pathways of post-replicational repair in uvrB strains of Escherichia coli K-12. Youngs, D.A., Smith, K.C. J. Bacteriol. (1976) [Pubmed]
  17. Influence of a uvrD mutation on survival and repair of X-irradiated Escherichia coli K-12 cells. van der Schueren, E., Youngs, D.A., Smith, K.C. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. (1977) [Pubmed]
  18. Effect of the uvrD mutation on excision repair. Kuemmerle, N.B., Masker, W.E. J. Bacteriol. (1980) [Pubmed]
  19. Developing a genetic system in Deinococcus radiodurans for analyzing mutations. Kim, M., Wolff, E., Huang, T., Garibyan, L., Earl, A.M., Battista, J.R., Miller, J.H. Genetics (2004) [Pubmed]
  20. The involvement of an E. coli multiprotein complex in the complete repair of UV-damaged DNA. Grossman, L., Caron, P.R., Oh, E.Y. Basic Life Sci. (1986) [Pubmed]
  21. Long repair replication patches are produced by the short-patch pathway in a uvrD252 (recL152) mutant of Escherichia coli K-12. Rothman, R.H., Fried, B. J. Bacteriol. (1984) [Pubmed]
  22. Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases. Colloms, S.D., Sykora, P., Szatmari, G., Sherratt, D.J. J. Bacteriol. (1990) [Pubmed]
  23. Indirect stimulation of recombination in Escherichia coli K-12: dependence on recJ, uvrA, and uvrD. Schellhorn, H.E., Low, K.B. J. Bacteriol. (1991) [Pubmed]
  24. lon incompatibility associated with mutations causing SOS induction: null uvrD alleles induce an SOS response in Escherichia coli. SaiSree, L., Reddy, M., Gowrishankar, J. J. Bacteriol. (2000) [Pubmed]
  25. Nucleotide sequence of the regulatory region of the uvrD gene of Escherichia coli. Finch, P., Emmerson, P.T. Gene (1983) [Pubmed]
  26. Antibacterial efficacy of cefazolin and cephradine in neutropenic mice. Kunst, M.W., Mattie, H., van Furth, R. Infection (1979) [Pubmed]
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