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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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The influence of messenger RNA secondary structure on expression of an immunoglobulin heavy chain in Escherichia coli.

A gene for murine mu heavy chain immunoglobulin has been inserted into a bacterial expression plasmid containing the Escherichia coli trp promoter and ribosome binding site. A low level expression of mu protein was detected. Secondary structure analysis showed the presence of a hairpin loop burying the mu initiation codon. Alteration of secondary structure at this site by oligonucleotide replacement mutagenesis revealed a correlation between mu expression levels and accessibility of the ribosome binding site. Abolition of secondary structure increased mu protein expression over ninety-fold, to a level approximately equal to that of a trpE -mu fusion protein using the native trpE ribosome binding site.[1]

References

  1. The influence of messenger RNA secondary structure on expression of an immunoglobulin heavy chain in Escherichia coli. Wood, C.R., Boss, M.A., Patel, T.P., Emtage, J.S. Nucleic Acids Res. (1984) [Pubmed]
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