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Gene Review

trpE  -  component I of anthranilate synthase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1258, JW1256, anth, tryD, tryp-4
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Disease relevance of trpE


High impact information on trpE

  • Restriction fragments of DNA derived from a cDNA clone of the alpha subunit of the acetylcholine receptor were subcloned in Escherichia coli by using the trpE fusion vector, pATH2 [6].
  • The v-abl oncogene was expressed in Escherichia coli as a trpE/v-abl fusion protein from the trp promoter [7].
  • A region in the skeletal muscle ryanodine receptor between amino acids 4014 and 4765 was expressed as a trpE fusion protein [8].
  • Using antibodies against synthetic peptides and trpE fusion proteins, we detected an 81-kDa protein encoded by this open reading frame in mitochondrial preparations from the plasmid-containing strains [9].
  • We have prepared fusion polypeptide genes from fragments of chloroplast DNA homologous to bacterial RNA polymerase subunit genes and expression vectors carrying portions of the anthranilate synthetase gene (trpE) [10].

Chemical compound and disease context of trpE


Biological context of trpE

  • The 5'-flanking region encodes two open reading frames (ORFs); one corresponds to trpL, while the other corresponds to the N-terminal half of the trpE gene [13].
  • This plasmid construct promotes a high level of expression of the trpE/COR1 hybrid protein [14].
  • The amino acid sequences, alpha 184-200 and alpha 184-196 of the Torpedo californica alpha-subunit of the nicotinic acetylcholine receptor were expressed as trpE fusion proteins via the expression vector pATH2 and a method for the enrichment of these fusion proteins is described [15].
  • The expression system was programmed with a plasmid containing a trpE-prfB fusion gene so that each of the products of the competing events could be measured [16].
  • Abolition of secondary structure increased mu protein expression over ninety-fold, to a level approximately equal to that of a trpE -mu fusion protein using the native trpE ribosome binding site [17].

Associations of trpE with chemical compounds


Other interactions of trpE


Analytical, diagnostic and therapeutic context of trpE


  1. Nucleotide sequence of Escherichia coli trpE. Anthranilate synthetase component I contains no tryptophan residues. Nichols, B.P., van Cleemput, M., Yanofsky, C. J. Mol. Biol. (1981) [Pubmed]
  2. Tryptophan biosynthesis genes trpEGC in the thermoacidophilic archaebacterium Sulfolobus solfataricus. Tutino, M.L., Scarano, G., Marino, G., Sannia, G., Cubellis, M.V. J. Bacteriol. (1993) [Pubmed]
  3. Analysis of adenovirus transforming proteins from early regions 1A and 1B with antisera to inducible fusion antigens produced in Escherichia coli. Spindler, K.R., Rosser, D.S., Berk, A.J. J. Virol. (1984) [Pubmed]
  4. Bacterially expressed nucleoprotein of infectious hematopoietic necrosis virus augments protective immunity induced by the glycoprotein vaccine in fish. Oberg, L.A., Wirkkula, J., Mourich, D., Leong, J.C. J. Virol. (1991) [Pubmed]
  5. Gene product of Moloney murine leukemia virus required for proviral integration is a DNA-binding protein. Roth, M.J., Tanese, N., Goff, S.P. J. Mol. Biol. (1988) [Pubmed]
  6. Expression of the alpha-bungarotoxin binding site of the nicotinic acetylcholine receptor by Escherichia coli transformants. Gershoni, J.M. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  7. Isolation of temperature-sensitive tyrosine kinase mutants of v-abl oncogene by screening with antibodies for phosphotyrosine. Kipreos, E.T., Lee, G.J., Wang, J.Y. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  8. Characterization of a Ca2+ binding and regulatory site in the Ca2+ release channel (ryanodine receptor) of rabbit skeletal muscle sarcoplasmic reticulum. Chen, S.R., Zhang, L., MacLennan, D.H. J. Biol. Chem. (1992) [Pubmed]
  9. Identification of the reverse transcriptase encoded by the Mauriceville and Varkud mitochondrial plasmids of Neurospora. Kuiper, M.T., Sabourin, J.R., Lambowitz, A.M. J. Biol. Chem. (1990) [Pubmed]
  10. Chloroplast rpoA, rpoB, and rpoC genes specify at least three components of a chloroplast DNA-dependent RNA polymerase active in tRNA and mRNA transcription. Little, M.C., Hallick, R.B. J. Biol. Chem. (1988) [Pubmed]
  11. Expression of Sulfolobus solfataricus trpE and trpG genes in E. coli. Tutino, M.L., Tosco, A., Marino, G., Sannia, G. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  12. Characterization of Bacillus caldotenax anthranilate synthase I produced in Escherichia coli and identification of its essential arginine residue by site-directed mutagenesis. Shiratsuchi, A., Sato, S. J. Biochem. (1992) [Pubmed]
  13. Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutamic-acid-producing bacterium. Sano, K., Matsui, K. Gene (1987) [Pubmed]
  14. Assembly of the mitochondrial membrane system. Characterization of COR1, the structural gene for the 44-kilodalton core protein of yeast coenzyme QH2-cytochrome c reductase. Tzagoloff, A., Wu, M.A., Crivellone, M. J. Biol. Chem. (1986) [Pubmed]
  15. Characterization of the binding of alpha-bungarotoxin to bacterially expressed cholinergic binding sites. Aronheim, A., Eshel, Y., Mosckovitz, R., Gershoni, J.M. J. Biol. Chem. (1988) [Pubmed]
  16. Competition between frameshifting, termination and suppression at the frameshift site in the Escherichia coli release factor-2 mRNA. Adamski, F.M., Donly, B.C., Tate, W.P. Nucleic Acids Res. (1993) [Pubmed]
  17. The influence of messenger RNA secondary structure on expression of an immunoglobulin heavy chain in Escherichia coli. Wood, C.R., Boss, M.A., Patel, T.P., Emtage, J.S. Nucleic Acids Res. (1984) [Pubmed]
  18. Studies of the hyperthermophile Thermotoga maritima by random sequencing of cDNA and genomic libraries. Identification and sequencing of the trpEG (D) operon. Kim, C.W., Markiewicz, P., Lee, J.J., Schierle, C.F., Miller, J.H. J. Mol. Biol. (1993) [Pubmed]
  19. Nucleotide sequence of Escherichia coli pabB indicates a common evolutionary origin of p-aminobenzoate synthetase and anthranilate synthetase. Goncharoff, P., Nichols, B.P. J. Bacteriol. (1984) [Pubmed]
  20. Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax. Shiratsuchi, A., Sato, S. Biochim. Biophys. Acta (1991) [Pubmed]
  21. Punctuation of transcription in vitro of the tryptophan operon of Escherichia coli. A novel type of control of transcription. Pannekoek, H., Brammar, W.J., Pouwels, P.H. Mol. Gen. Genet. (1975) [Pubmed]
  22. Epitope mapping and characterization of the infectious hematopoietic necrosis virus glycoprotein, using fusion proteins synthesized in Escherichia coli. Xu, L., Mourich, D.V., Engelking, H.M., Ristow, S., Arnzen, J., Leong, J.C. J. Virol. (1991) [Pubmed]
  23. Molecular cloning and nucleotide sequence of Thermus thermophilus HB8 trpE and trpG. Sato, S., Nakada, Y., Kanaya, S., Tanaka, T. Biochim. Biophys. Acta (1988) [Pubmed]
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