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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Partial purification and characterization of arachidonate 12-lipoxygenase from rat lung.

Incubation of rat lung cytosol with arachidonic acid produced 12-hydroxy-5,8,10,14-eicosatetraenoic acid as a major product, which was identified by gas chromatography-mass spectrometry. By ammonium sulfate fractionation and DEAE-cellulose chromatography the arachidonate 12-lipoxygenase was purified about 30-fold from the rat lung cytosol. The partially purified enzyme was mostly free of the glutathione peroxidase activity and transformed arachidonic acid to its 12-hydroperoxide. 5,8,11,14,17-Eicosapentaenoic acid was also an active substrate, and the oxygenation at C-12 was confirmed by mass spectrometry. A significant amount of 12-lipoxygenase activity was also found in the microsomes and other particulate fractions.[1]

References

  1. Partial purification and characterization of arachidonate 12-lipoxygenase from rat lung. Yokoyama, C., Mizuno, K., Mitachi, H., Yoshimoto, T., Yamamoto, S., Pace-Asciak, C.R. Biochim. Biophys. Acta (1983) [Pubmed]
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