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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Dehydroascorbate uptake as an in vitro biochemical marker of granulocyte differentiation.

We tested the hypothesis that the rate of cellular uptake of dehydroascorbate in cultures of developing granulocyte-macrophage progenitors in vitro would serve as a biochemical marker of neutrophil maturation. Suspension cultures of low-density, nonadherent, T-lymphocyte-depleted bone marrow cells from eight normal volunteers were cultured in medium containing 10% human placental conditioned medium and were harvested at intervals over 14 days. The harvested cells were tested for their ability to take up dehydroascorbate. Mean cellular uptake rate increased 12-fold by Day 10, at which time the cells had differentiated to neutrophils. Uptake increased by less than 2-fold in cells which had been induced to differentiate to mature mononuclear phagocytes with 12-O-tetradecanoylphorbol-13-acetate. Additional studies using HL-60 cells induced to differentiate with dimethyl sulfoxide or 12-O-tetradecanoylphorbol-13-acetate support the view that a major increase in dehydroascorbate uptake in cultured granulopoietic progenitors is a manifestation of a neutrophil differentiation.[1]


  1. Dehydroascorbate uptake as an in vitro biochemical marker of granulocyte differentiation. Anderson, R., Stankova, L., Bigley, R.H., Bagby, G.C. Cancer Res. (1983) [Pubmed]
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