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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A rapid assay for protein kinases phosphorylating small polypeptides and other substrates.

A new and rapid method of protein kinase assay is presented which is suitable for low-molecular-weight substrates, irrespective of their electrophoretic or chromatographic mobility. It depends on the phosphorylation of the substrates with [gamma-32P]ATP, hydrolysis of the pyrophosphate bonds by boiling in 1 N HCl, extraction of 32P with isobutanol-benzene, and measurement of the radioactivity of 32P-labeled phosphoesters in the water phase. The method is shown to be suitable for both tyrosine- and serine-phosphorylating protein kinases.[1]

References

  1. A rapid assay for protein kinases phosphorylating small polypeptides and other substrates. Braun, S., Abdel Ghany, M., Racker, E. Anal. Biochem. (1983) [Pubmed]
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