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Radioactive labeling of antibody: a simple and efficient method.

A simple and efficient method of covalently coupling the strong chelator diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling immunoglobulin G antibodies. After being coupled and labeled with indium-111, a monoclonal antibody to carcinoembryonic antigen retained its ability to bind to its antigen in vitro and in vivo. In nude mice with a human colorectal xenograft, 41 percent of the injected radioactivity became localized in each gram of xenograft at 24 hours compared with 9 percent for control antibody and 19 percent for radioiodinated antibody to carcinoembryonic antigen.[1]


  1. Radioactive labeling of antibody: a simple and efficient method. Hnatowich, D.J., Layne, W.W., Childs, R.L., Lanteigne, D., Davis, M.A., Griffin, T.W., Doherty, P.W. Science (1983) [Pubmed]
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