The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Oligomerization of malate synthase during glyoxysome biosynthesis.

The octameric malate synthase, found in glyoxysomes of plants, is synthesized as monomeric precursor in the cytoplasm. The precursor form does not possess a different subunit molecular weight than the mature organellar enzyme, but differs from the organellar protein by not oligomerizing and aggregating. This was shown by synthesis in a cell-free reticulocyte lysate system programmed with cucumber poly A+-mRNA followed by immunoprecipitation of the radiolabeled translation products. The precursor form of malate synthase was also detected in vivo in the cytosol of pulse-labeled cucumber cotyledons after immunoprecipitation of the radiolabeled polypeptide. At low salt concentrations, mature malate synthase can be converted into aggregated forms. However, the precursor form obtained either by in vitro translation or by extraction from the cytosol after short pulses of radioactive methionine, could neither be oligomerized into the octameric form nor aggregated into the 100-S form. Processing of malate synthase, assumed to be a requisite for oligomerization, took place rapidly in the glyoxysomes, but proceeded only slowly in the cytosol. This was demonstrated both by the uptake of in vitro-translated malate synthase into glyoxysomes, and by analysis of newly synthesized malate synthase detectable in glyoxysomes in vivo. In both cases the octamer was by far the predominant form.[1]


  1. Oligomerization of malate synthase during glyoxysome biosynthesis. Kruse, C., Kindl, H. Arch. Biochem. Biophys. (1983) [Pubmed]
WikiGenes - Universities