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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Measurement of cholesterol 7 alpha-hydroxylase activity with selected ion monitoring.

Formation of 7 alpha-hydroxycholesterol by rat liver microsomes was quantitated using a gas chromatograph-mass spectrometer operated in selected ion monitoring mode. This procedure measures the mass of 7 alpha-hydroxycholesterol formed and does not require addition of labeled cholesterol to monitor the reaction, since endogenous microsomal cholesterol serves as substrate for the enzyme cholesterol 7 alpha-hydroxylase. The method requires only the extraction of lipids from microsomal incubations and injection of a small fraction of this extract into the apparatus. Microsomes from normal rat livers incubated for different periods were found to yield increased 7 alpha-hydroxycholesterol with time.[1]

References

  1. Measurement of cholesterol 7 alpha-hydroxylase activity with selected ion monitoring. Sanghvi, A., Grassi, E., Bartman, C., Lester, R., Galli Kienle, M., Galli, G. J. Lipid Res. (1981) [Pubmed]
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