beta-1,3-Glucanase is highly-expressed in laticifers of Hevea brasiliensis.
Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleotide homology to gnl cDNA. Northern blot analysis using the Hevea cDNA as probe detected a mRNA of 1.3 kb which was expressed at higher levels in latex than in leaf. In situ hybridization analysis using petiole sections from Hevea localized the beta-1,3-glucanase mRNA to the laticifer cells. Genomic Southern analysis suggested the presence of a low-copy gene family encoding beta-1,3-glucanases in H. brasiliensis.[1]References
- beta-1,3-Glucanase is highly-expressed in laticifers of Hevea brasiliensis. Chye, M.L., Cheung, K.Y. Plant Mol. Biol. (1995) [Pubmed]
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