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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Molecular cloning of the human angiotensin II type 2 receptor cDNA.

A human adult lung cDNA library was screened and one full-length human angiotensin II type 2 receptor (hAT2R) clone was isolated and characterized. The hAT2R cDNA clone contains a 1089-base-pair open reading frame which encodes a protein of 363 amino acid residues. The hAT2R is approximately 92% identical in sequence to the rat and mouse AT2R sequences. Specific binding of [125I]CGP42112A was demonstrated in membranes from COS-7 cells transiently transfected with the hAT2R cDNA. Scatchard analysis and ligand displacement profiles were typical of the AT2R. Northern analysis demonstrated that the hAT2R mRNA was abundantly expressed in human adult lung and in human fetal kidney. Additionally, the hAT2R mRNA was just detectable in human adult heart and aorta. In contrast, the rat AT2R mRNA was abundantly expressed in the rat brain and just detectable in the rat lung.[1]

References

  1. Molecular cloning of the human angiotensin II type 2 receptor cDNA. Martin, M.M., Su, B., Elton, T.S. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
 
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