Characterization of haemolyser-resistant cells increased in the blood of erythropoietin-treated mice.
Recently, we reported a new in vivo assay method for erythropoietin ( Epo) by means of counts of the number of haemolyser-resistant cells (HRCs) increased in Epo-injected mice. Here, we attempted to characterize the HRCs. Flow-cytometric studies revealed that HRCs obtained from Epo-injected mice expressed the transferrin receptor on their surface membranes. Furthermore, a fluorophotometric study suggested that the number of transferrin receptor-positive cells increased in a dose-dependent manner in response to treatment with Epo. On the other hand, flowcytometric and fluorophotometric studies of glycophorin A on HRCs using a rabbit antiglycophorin A antibody also showed a high expression of glycophorin A on them as compared with on HRCs from vehicle-treated animals (control). The results indicated that HRCs could be defined by their expression of both transferrin receptors and glycophorin A. We concluded that HRCs might be immature reticulocytes.[1]References
- Characterization of haemolyser-resistant cells increased in the blood of erythropoietin-treated mice. Said, A.A., Yamaguchi, T., Uchida, E., Hayakawa, T. Br. J. Haematol. (1994) [Pubmed]
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