Use of monoclonal antibodies to isolate and characterize Cyn d I, the major allergen of Bermuda grass pollen.
BACKGROUND: Cyn d I has been found to be the major allergen of Bermuda grass (Cynodon dactylon) pollen, but its exact nature remains to be clarified. METHODS: Cyn d I, the major allergen of Bermuda grass (Cynodon dactylon) pollen, was purified by monoclonal antibody (MoAb) affinity chromatography, and its biochemical and immunologic properties were characterized. Anti-Cyn d I MoAb 4-37, which recognizes all of the isoallergens of Cyn d I, was chosen as the immunosorbent. RESULTS: The purified protein has an amino acid composition similar to that of the group I allergens of other grass pollens. It appears as a single 34 kd band or as a mixture of 34 and 29 kd polypeptides in sodium dodecylsulfate-polyacrylamide gel electrophoresis analysis. The hydrophobicity of these two polypeptides is similar because they have the same retention time on a C18 reverse-phase column when a trifluoroacetic acid/H2O/CH3CN buffer system is used. The N-terminal amino acid sequence of the 34 kd component has a 60% homology with residues of 1-25 of Lol p I, whereas that of the 29 kd component has a 68% homology with residues 31-68 of Lol p I. In addition, this 29 kd polypeptide can be recognized by another anti-Cyn d I MoAb 1-61. CONCLUSIONS: These results suggest that the 29 kd component is derived from Cyn d I. In spite of the similarity in the amino acid composition between Cyn d I and group I allergens of other grass pollens, none of our four anti-Cyn d I MoAbs cross-reacted with 10 other grass pollens tested, including ryegrass pollen. Despite biochemical similarity with other group I allergens, the B-cell epitopes on Cyn d I are different from those on other grass pollens.[1]References
- Use of monoclonal antibodies to isolate and characterize Cyn d I, the major allergen of Bermuda grass pollen. Han, S.H., Chang, Z.N., Chi, C.W., Perng, H.J., Liu, C.C., Tsai, J.J., Tam, M.F. J. Allergy Clin. Immunol. (1993) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
