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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Partial structure of the mouse glucokinase gene.

A complementary DNA for glucokinase (GK) was cloned from mouse liver total RNA by a combination of the polymerase chain reaction (PCR) and mouse liver cDNA library screening. Liver- and beta-cell-specific exons 1 were isolated by PCR using mouse and rat genomic DNAs. These clones were then used to screen a mouse genomic library; three genomic clones were isolated and characterized. The mouse GK gene spans over 20 kb, containing 11 exons including a liver- or beta-cell-specific exon 1, which encodes a tissue-specific 15-aa peptide at the N-terminus of the protein. Both types of GK contain 465 amino acid residues. The predicted amino acid sequence of mouse beta-cell-specific GK showed 98 and 96% identity to the rat and human enzymes, respectively; the corresponding values are 98 and 95%, respectively, for the liver-specific GK. Several transcription factor-binding consensus sequences are identified in the 5' flanking region of the mouse GK gene.[1]

References

  1. Partial structure of the mouse glucokinase gene. Ishimura-Oka, K., Nakamuta, M., Chu, M.J., Sullivan, M., Chan, L., Oka, K. Genomics (1995) [Pubmed]
 
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