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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Mapping of murine YACs containing the genes Cea2 and Cea4 after B1-PCR amplification and FISH-analysis.

PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescence in situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomal in situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes.[1]

References

  1. Mapping of murine YACs containing the genes Cea2 and Cea4 after B1-PCR amplification and FISH-analysis. Rettenberger, G., Zimmermann, W., Klett, C., Zechner, U., Hameister, H. Chromosome Res. (1995) [Pubmed]
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