Meiotic cell cycle requirement for a fly homologue of human Deleted in Azoospermia.
Infertility resulting from a severe defect in sperm production affects 2% of men worldwide. Of these men with azoospermia, the absence of sperm in semen, one in eight carry de novo deletions for a specific region of the Y chromosome. A candidate gene for the Y-chromosome azoospermia factor ( AZF) has been identified and named Deleted in Azoospermia ( DAZ). Here we describe the cloning and characterization of the Drosophila gene boule, which is a homologue of DAZ. The two genes encode closely related proteins that contain a predicted RNA-binding motif, and both loci are expressed exclusively in the testis. Loss of boule function results in azoospermia; meiotic divisions are blocked, although limited spermatid differentiation occurs. Histological examination of boule testes with cell-cycle markers indicates that the primary defect is at the meiotic G2/M transition. These results support the hypothesis that DAZ is the human AZF, and indicate that Boule and DAZ have an essential meiotic function in fly and human spermatogenesis.[1]References
- Meiotic cell cycle requirement for a fly homologue of human Deleted in Azoospermia. Eberhart, C.G., Maines, J.Z., Wasserman, S.A. Nature (1996) [Pubmed]
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