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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Serologic responses of cattle and other animals infected with Neospora caninum.

OBJECTIVE--To examine cross-reactivity among Neospora caninum and closely-related apicomplexans. DESIGN--Sera from animals were examined for antibody production to N caninum and cross-reactivity to Toxoplasma gondii. ANIMALS--Cattle were experimentally infected with 3 tissue cyst-forming protozoan parasites N caninum, T gondii, and Sarcocystis sp, and calves were monospecifically inoculated with the intestinal coccidia, Eimeria bovis and Cryptosporidium parvum. Similar studies were done in laboratory rabbits inoculated with N caninum, T gondii, Hammondia hammondi, and Sarcocystis sp. Additionally, sera were obtained from ewes, lambs, goats, sows, cats, rats, and mice inoculated with N caninum tachyzoites. PROCEDURE--The indirect fluorescent antibody (IFA) and ELISA antibody tests (cattle only) were used to examine reactivity to N caninum; the modified direct agglutination, Sabin-Feldman dye, and IFA tests were used to evaluate reactivity to T gondii. RESULTS--Serologic cross-reactivity among N caninum, T gondii, and Sarcocystis sp was none or minimal by the IFA test. There was some reactivity to N caninum by the use of ELISA in cattle inoculated with Sarcocystis sp. CONCLUSIONS--The IFA test for N caninum was specific for the diagnosis of neosporosis in animals.[1]


  1. Serologic responses of cattle and other animals infected with Neospora caninum. Dubey, J.P., Lindsay, D.S., Adams, D.S., Gay, J.M., Baszler, T.V., Blagburn, B.L., Thulliez, P. Am. J. Vet. Res. (1996) [Pubmed]
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