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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Cryopreservation of rooster semen in thirteen and sixteen percent glycerol.

Semen from Barred Plymouth Rock roosters was cryopreserved with glycerol concentrations of 13 and 16% in a microprocessor-controlled freezer. Thawing and deglycerolation were facilitated by the use of an improved microprocessor-controlled thawing device and high speed dialyzer. Deglycerolated semen (100 mu L; 192 and 154 million sperm, respectively, for the 13 and 16% glycerol concentration) was inseminated into Single Comb white Leghorn hens. Three inseminations were done at 4-d intervals. Eggs were collected for 10 d starting 1 d after the first insemination, and incubated for 4th d. Fertility was determined by candling after the 4th d. Fertility measurements of 62.4 and 65% were obtained from the sperm frozen in 13 and 16% glycerol concentrations, respectively, for the 10-d period.[1]


  1. Cryopreservation of rooster semen in thirteen and sixteen percent glycerol. Gill, S.P., Buss, E.G., Mallis, R.J. Poult. Sci. (1996) [Pubmed]
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