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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Overexpression of the gene for N-acylamino acid racemase from Amycolatopsis sp. TS-1-60 in Escherichia coli and continuous produciton of optically active methionine by a bioreactor.

A DNA sequence encoding N-acylamino acid racemase (AAR) was inserted downstream from the T7 promoter in pET3c. The recombinant plasmid was introduced into Escherichia coli MM 294 lysogenized with a bacteriophage lambda having a T7 RNA polymerase gene. The amount of AAR produced by the E. coli transformant was 1100-fold more than that produced by Amycolatopsis sp. TS-1-60, the DNA donor strain. The AAR was purified to homogeneity from the crude extract of the E. coli transformant by two steps: heat treatment and Butyl-Toyopearl column chromatography. Bioreactors for the production of optically active amino acids were constructed with DEAE-Toyopearl-immobilized AAR and D- or L-aminoacylase. D- or L-methionine was continuously produced with a high yield from N-acetyl-DL-methionine by the bioreactor.[1]

References

  1. Overexpression of the gene for N-acylamino acid racemase from Amycolatopsis sp. TS-1-60 in Escherichia coli and continuous produciton of optically active methionine by a bioreactor. Tokuyama, S., Hatano, K. Appl. Microbiol. Biotechnol. (1996) [Pubmed]
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