Disease relevance of NSC80751

• N-Acetylmethionine (NAM) has proven to be an effective antidote against BB toxicity when given after liver GSH has been depleted extensively [1].
• L-Methionine at levels of 1.2% and above, or equivalent levels of N-acetyl-L-methionine caused comparable hypertrophy of the spleen and comparable increases in spleen iron levels [2].
• N-acetyl-D-methionine in excess depressed growth only slightly, due entirely to a depression in voluntary food intake, and no evidence of splenic hemosiderosis was observed from this addition [3].
• Sequence analyses of the cDNA and several Achromobacter protease I-digested peptides of the purified protein revealed that porcine liver AARE consists of four identical subunits, and each comprising a single chain of 732 amino acids with acetylmethionine at the N-terminus [4].
• Although the amino-end of the amplified TS was unblocked, that isolated from a FUdR-resistant rat hepatoma cell line contained mostly N-acetylmethionine on its N-terminal end, a finding that may have significant regulatory consequences, which are discussed [5].

High impact information on NSC80751

• The presence of a cysteine residue(s) near the active site of acylpeptide hydrolase was suggested by inactivation of the enzyme with sulfhydryl-modifying agents and by the substantial protection against inactivation afforded by the competitive inhibitor acetylmethionine [6].
• Enzyme inactivation, which follows pseudo first-order kinetics, is diminished by the competitive inhibitor acetylmethionine [6].
• However, there are some class II actins in which this residue is other than cysteine, suggesting a broader substrate specificity for actin N-acetylaminopeptidase than acetylmethionine or acetylcysteine [7].
• The amino-terminal residue of GAL80 was found to be acetylmethionine [8].
• Thionein-I is a single chain polypeptide which consists of 61 amino acid residues and has the amino acid composition Cys20, Asp3, Asn1, Thr5, Ser9, Gln1, Pro2, Gly5, Ala5, Val2, Met1, Lys7, with N-acetylmethionine and alanine as NH2- and COOH-terminal amino acids, respectively [9].

Chemical compound and disease context of NSC80751

• Chick growth assays were used to evaluate the efficacy and toxicity of the L- and D-isomers of N-acetylmethionine when fed as a source of methionine in crystalline amino acid diets [3].

Biological context of NSC80751

• Gastric emptying did not account for this difference apparently reflecting a slower rate of absorption of N-acetyl-L-methionine by mucosal cells [10].
• Maximal aminoacylase activity with N-acetyl-L-methionine as the substrate occurred at pH 6.5 and a temperature of 100 degrees C. The N-terminal amino acid sequence of the purified aminoacylase was used to identify, in the P. furiosus genome database, a gene that encodes 383 amino acids [11].
• Covalent attachment of amino acids to casein. 2. Bioavailability of methionine and N-acetylmethionine covalently linked to casein [12].
• The kinetic and thermodynamic parameters of the hog kidney acylase-catalyzed reactions of N-acetyl-L-methionine hydrolysis and synthesis have been investigated [13].

Anatomical context of NSC80751

• A peptide initiation factor purified from rat liver and promoting the binding of initiator tRNA and model initiators to 40S and 80S ribosome at an acid pH liberates methionine and N-acetylmethionine from Trna Met f at neutral reaction [14].
• Levels of NAARE exceeded acylase measured with acetylmethionine in all brain regions and subcellular fractions examined: 60% was associated with cytosol and the remainder with debris or the crude nuclear and mitochondrial-synaptosomal subfractions [15].
• Plasma and erythrocyte methionine levels and the area under the plasma and erythrocyte methionine absorption curve were compared in five normal adult subjects administered equimolar quantities (0.0605 mmoles/kg) of L-methionine and N-acetyl-L-methionine [10].
• 1. Human adenylate kinase (isoenzyme AK-1-1) from skeletal muscle is a single polypeptide chain of 194 amino-acid residues with an acetylmethionine at the N-terminus and a lysine at the C-terminus [16].
• Both Suc-Ala-Ala-Phe-4 methoxy-2-naphthylamide (MNA) and N-acetyl-L-methionine-alpha-naphthyl ester (alpha-N-O-Met) reacted with chymase but not tryptase in mast cells [17].

Associations of NSC80751 with other chemical compounds

• The compounds were assayed for their abilities to function as acetyl donors in the AHAT-mediated transacetylation of 4-aminoazobenzene and for their abilities to participate in the AHAT-mediated conversion of N-arylhydroxylamines to electrophilic intermediates that form methylthio adducts upon reaction with N-acetylmethionine [18].
• Following cleavage with thrombin and purification, the soluble 24 kDa protein showed MsrA activity with N-acetylmethionine sulphoxide as substrate, as well as with other sulphoxide compounds [19].
• PAM compares favourably in hepatoprophylactic effect, to concomitant administration of equimolar doses of free N-acetyl-DL-methionine added to the paracetamol formulation [20].
• Incubation of catechol with mushroom tyrosinase in the presence of N-acetylmethionine resulted in the generation of an adduct [21].
• Nevertheless, occurrence of this nonenzymatic reaction between N-acetylmethionine and mushroom tyrosinase-generated quinones indicates that a similar reaction between enzymatically generated quinones in the cuticle with protein-bound methionine moiety is likely to occur during in vivo quinone tanning as well [21].

Gene context of NSC80751

• The r alpha-1-AT has a molecular weight of 45,000, no carbohydrates, and is identical in sequence to normal plasma alpha-1-AT except for an additional N-terminal acetylmethionine [22].
• The expressed NAAAR racemized various substrates at an optimal temperature of 60 degrees C and had Km values of 24.8 mM and 12.3 mM for N-acetyl-D-methionine and N-acetyl-L-methionine, respectively [23].
• Glucosamine and acetylmethionine, but not acetyl glucosamine, diminished the intestinal transfer of tetracyclines [24].
• The N-terminal residue of bovine rhodopsin is acetylmethionine [25].
• All four bands of the esterase A2 preparation had enzyme activity since all were stainable on zymograms using N-acetyl-L-methionine alpha-naphthyl ester as substrate [26].

Analytical, diagnostic and therapeutic context of NSC80751

• D- or L-methionine was continuously produced with a high yield from N-acetyl-DL-methionine by the bioreactor [27].

References