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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene.

Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse polymerase chain reaction (PCR). The cDNA codes for a 396 amino-acid protein with two potential trans-membrane domains. Y. lipolytica Sec62p behaves as an integral membrane protein as shown by Western blotting. Y. lipolytica SEC62 cDNA is able to complement a S. cerevisiae sec62 null mutant strain confirming functional conservation, although only 53.6% amino-acid similarity is observed between Y. lipolytica and S. cerevisiae Sec62.[1]

References

  1. Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene. Swennen, D., Joyet, P., Gaillardin, C. Curr. Genet. (1997) [Pubmed]
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