Membrane type-1 matrix metalloproteinase in human ocular tissues.
PURPOSE: Membrane type-1 matrix metalloproteinase ( MT1-MMP) ( MMP-14) (EC 3.4.24.xx) is involved in the activation of progelatinase A ( MMP-2) (EC 3.4.24.24). MMP-2 is present at least in the interphotoreceptor matrix and vitreous. The purpose of this study was to determine the distribution of MT1-MMP, and MMP-2, in human ocular tissues. METHODS: The distribution of MT1-MMP and MMP-2 was investigated in vitreous and in membrane extracts from eye tissues obtained from postmortem human eyebank eyes. Western blot analysis was performed using mouse monoclonal anti- MT1-MMP and anti-MMP-2 antibodies. RESULTS: MT1-MMP was found in sclera, cornea, lens, choroid, retinal pigment epithelium (RPE) and retina. MMP-2 was found in sclera, cornea, choroid, vitreous, RPE and retina but was absent from lens. CONCLUSION: We provide the first evidence for the presence and distribution of membrane-type-1 matrix metalloproteinase in human ocular tissues. MT1-MMP may be responsible for the activation of progelatinase A in many ocular extracellular matrices in a manner similar to that exhibited in other systems.[1]References
- Membrane type-1 matrix metalloproteinase in human ocular tissues. Smine, A., Plantner, J.J. Curr. Eye Res. (1997) [Pubmed]
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