Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Ishii, M. et al.

Expression and clustered distribution of an inwardly rectifying potassium channel, KAB-2/Kir4.1, on mammalian retinal MÃ¼ller cell membrane: their regulation by insulin and laminin signals.

Inwardly rectifying potassium (K+) channels (Kir) in MÃ¼ller cells, the dominant glial cells in the retina, are supposed to be responsible for the spatial buffering action of K+ ions. The molecular properties and subcellular localization of MÃ¼ller cell Kir channels in rat and rabbit retinas were examined by using electrophysiological, molecular biological, and immunostaining techniques. Only a single population of Kir channel activity, the properties of which were identical to those of KAB-2/Kir4.1 expressed in HEK293T cells, could be recorded from endfoot to the distal portion of MÃ¼ller cells. Consistently, Northern blot, in situ hybridization, and RT-PCR analyses indicated expression of Kir4. 1 in MÃ¼ller cells per se. The Kir4.1 immunoreactivity was distributed in clusters throughout MÃ¼ller cell membrane. The Kir4.1 expression in MÃ¼ller cells disappeared promptly after culturing. When the dissociated MÃ¼ller cells were cultured on laminin-coated dishes in the presence of insulin, Kir4.1 immunoreactivity was detected in a clustered manner on the cell membrane. Because insulin and laminin exist in the surrounding of MÃ¼ller cells in the retina, these substances possibly may be physiological regulators of expression and distribution of Kir4.1 in MÃ¼ller cells in vivo.[1]

References

Expression and clustered distribution of an inwardly rectifying potassium channel, KAB-2/Kir4.1, on mammalian retinal Müller cell membrane: their regulation by insulin and laminin signals. Ishii, M., Horio, Y., Tada, Y., Hibino, H., Inanobe, A., Ito, M., Yamada, M., Gotow, T., Uchiyama, Y., Kurachi, Y. J. Neurosci. (1997) [Pubmed]

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