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Berndt, C. et al.

Berndt, Haubold, Wenger, Brux, Müller, Bendzko, Hillebrand, Köttgen, Zanow,

K-ras mutations in stools and tissue samples from patients with malignant and nonmalignant pancreatic diseases.

Mutant-enriched PCR and reverse dot blot hybridization in microplates were applied for examining K-ras status in stools and tissue samples from patients with pancreatic tumors and chronic pancreatitis. In tissue samples, K-ras mutations were found in 32 of 35 cases of ductal adenocarcinoma, in 5 of 7 periampullary cancers, in 1 cystadenocarcinoma, and in 3 of 5 patients with chronic pancreatitis. In stools, mutated K-ras was seen in 10 of 25 cases of ductal adenocarcinoma, in 1 case of cystadenocarcinoma, and in 2 of 6 cases of chronic pancreatitis. These data indicate that the K-ras status of stool samples may help identify pancreatic carcinoma and persons at risk for cancer development; however, it does not allow discrimination of malignant from nonmalignant diseases.[1]

References

K-ras mutations in stools and tissue samples from patients with malignant and nonmalignant pancreatic diseases. Berndt, C., Haubold, K., Wenger, F., Brux, B., Müller, J., Bendzko, P., Hillebrand, T., Köttgen, E., Zanow, J. Clin. Chem. (1998) [Pubmed]

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