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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects.

Yeast phosphatidylinositol transfer protein (Sec14p) function is essential for production of Golgi-derived secretory vesicles, and this requirement is bypassed by mutations in at least seven genes. Analyses of such 'bypass Sec14p' mutants suggest that Sec14p acts to maintain an essential Golgi membrane diacylglycerol (DAG) pool that somehow acts to promote Golgi secretory function. SPO14 encodes the sole yeast phosphatidylinositol-4,5-bisphosphate-activated phospholipase D (PLD). PLD function, while essential for meiosis, is dispensable for vegetative growth. Herein, we report specific physiological circumstances under which an unanticipated requirement for PLD activity in yeast vegetative Golgi secretory function is revealed. This PLD involvement is essential in 'bypass Sec14p' mutants where normally Sec14p-dependent Golgi secretory reactions are occurring in a Sec14p-independent manner. PLD catalytic activity is necessary but not sufficient for 'bypass Sec14p', and yeast operating under 'bypass Sec14p' conditions are ethanol-sensitive. These data suggest that PLD supports 'bypass Sec14p' by generating a phosphatidic acid pool that is somehow utilized in supporting yeast Golgi secretory function.[1]


  1. Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects. Xie, Z., Fang, M., Rivas, M.P., Faulkner, A.J., Sternweis, P.C., Engebrecht, J.A., Bankaitis, V.A. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
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