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Gene Review

SPO14  -  phospholipase D

Saccharomyces cerevisiae S288c

Synonyms: Choline phosphatase 1, Meiosis-specific sporulation-specific protein 14, PLD 1, PLD1, Phosphatidylcholine-hydrolyzing phospholipase D1, ...
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Disease relevance of SPO14


High impact information on SPO14


Biological context of SPO14


Anatomical context of SPO14


Associations of SPO14 with chemical compounds


Physical interactions of SPO14

  • Here we show that PLD/Spo14 forms a complex with Sma1, a meiotically expressed protein essential for spore formation [18].

Co-localisations of SPO14

  • Consistent with these findings, Sfh2p colocalizes with PLD in endosomal compartments [9].

Regulatory relationships of SPO14

  • The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p [10].

Other interactions of SPO14

  • Spo14 localized normally to the developing prospore membrane in arf1-myc arf2 mutants; however, the synthesis of the membrane was attenuated [19].
  • Thus, rather than a role for PtdOH in stimulating PtdIns(4,5)P2 formation, our findings indicate that during sporulation, Spo14p-mediated PtdOH production functions downstream of Sec14p-, Pik1p-, and Mss4p-dependent PtdIns(4,5)P2 synthesis [17].
  • Only gcs1delta mutants display a sporulation defect similar to that of spo14 mutants: cells deleted for GCS1 initiate the sporulation program but are defective in synthesis of the prospore membrane [10].
  • Sfh2-mediated suppression of inadequate Gcs1-4 function depended on phospholipase D, whereas inadequate Gcs1-4 activity was relieved by increasing levels of diacylglycerol (DAG) [20].
  • Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis [18].

Analytical, diagnostic and therapeutic context of SPO14


  1. Differential partitioning of lipids metabolized by separate yeast glycerol-3-phosphate acyltransferases reveals that phospholipase D generation of phosphatidic acid mediates sensitivity to choline-containing lysolipids and drugs. Zaremberg, V., McMaster, C.R. J. Biol. Chem. (2002) [Pubmed]
  2. Mutagenesis of phospholipase D defines a superfamily including a trans-Golgi viral protein required for poxvirus pathogenicity. Sung, T.C., Roper, R.L., Zhang, Y., Rudge, S.A., Temel, R., Hammond, S.M., Morris, A.J., Moss, B., Engebrecht, J., Frohman, M.A. EMBO J. (1997) [Pubmed]
  3. Activation of phospholipase D is an early event in integrin-mediated signalling leading to phagocytosis in human neutrophils. Serrander, L., Fällman, M., Stendahl, O. Inflammation (1996) [Pubmed]
  4. Interactions among pathways for phosphatidylcholine metabolism, CTP synthesis and secretion through the Golgi apparatus. Kent, C., Carman, G.M. Trends Biochem. Sci. (1999) [Pubmed]
  5. Activation of phospholipase D by the small GTPase Sar1p is required to support COPII assembly and ER export. Pathre, P., Shome, K., Blumental-Perry, A., Bielli, A., Haney, C.J., Alber, S., Watkins, S.C., Romero, G., Aridor, M. EMBO J. (2003) [Pubmed]
  6. Identification of a phosphoinositide binding motif that mediates activation of mammalian and yeast phospholipase D isoenzymes. Sciorra, V.A., Rudge, S.A., Prestwich, G.D., Frohman, M.A., Engebrecht, J., Morris, A.J. EMBO J. (1999) [Pubmed]
  7. Relocalization of phospholipase D activity mediates membrane formation during meiosis. Rudge, S.A., Morris, A.J., Engebrecht, J. J. Cell Biol. (1998) [Pubmed]
  8. A role for phospholipase D (Pld1p) in growth, secretion, and regulation of membrane lipid synthesis in yeast. Sreenivas, A., Patton-Vogt, J.L., Bruno, V., Griac, P., Henry, S.A. J. Biol. Chem. (1998) [Pubmed]
  9. Identification of a novel family of nonclassic yeast phosphatidylinositol transfer proteins whose function modulates phospholipase D activity and Sec14p-independent cell growth. Li, X., Routt, S.M., Xie, Z., Cui, X., Fang, M., Kearns, M.A., Bard, M., Kirsch, D.R., Bankaitis, V.A. Mol. Biol. Cell (2000) [Pubmed]
  10. The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p. Connolly, J.E., Engebrecht, J. Eukaryotic Cell (2006) [Pubmed]
  11. Commitment to meiosis in Saccharomyces cerevisiae: involvement of the SPO14 gene. Honigberg, S.M., Conicella, C., Espositio, R.E. Genetics (1992) [Pubmed]
  12. Nonclassical PITPs activate PLD via the Stt4p PtdIns-4-kinase and modulate function of late stages of exocytosis in vegetative yeast. Routt, S.M., Ryan, M.M., Tyeryar, K., Rizzieri, K.E., Mousley, C., Roumanie, O., Brennwald, P.J., Bankaitis, V.A. Traffic (2005) [Pubmed]
  13. Characterization of a regulated form of phospholipase D in the yeast Saccharomyces cerevisiae. Ella, K.M., Dolan, J.W., Meier, K.E. Biochem. J. (1995) [Pubmed]
  14. Phospholipase D: a lipid centric review. Jenkins, G.M., Frohman, M.A. Cell. Mol. Life Sci. (2005) [Pubmed]
  15. Identification of a novel, Ca(2+)-dependent phospholipase D with preference for phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae. Mayr, J.A., Kohlwein, S.D., Paltauf, F. FEBS Lett. (1996) [Pubmed]
  16. The role and relevance of phospholipase D1 during growth and dimorphism of Candida albicans. Hube, B., Hess, D., Baker, C.A., Schaller, M., Schäfer, W., Dolan, J.W. Microbiology (Reading, Engl.) (2001) [Pubmed]
  17. Roles of phosphoinositides and of Spo14p (phospholipase D)-generated phosphatidic acid during yeast sporulation. Rudge, S.A., Sciorra, V.A., Iwamoto, M., Zhou, C., Strahl, T., Morris, A.J., Thorner, J., Engebrecht, J. Mol. Biol. Cell (2004) [Pubmed]
  18. Differential requirement for phospholipase D/Spo14 and its novel interactor Sma1 for regulation of exocytotic vesicle fusion in yeast meiosis. Riedel, C.G., Mazza, M., Maier, P., Körner, R., Knop, M. J. Biol. Chem. (2005) [Pubmed]
  19. ADP-Ribosylation factors do not activate yeast phospholipase Ds but are required for sporulation. Rudge, S.A., Cavenagh, M.M., Kamath, R., Sciorra, V.A., Morris, A.J., Kahn, R.A., Engebrecht, J. Mol. Biol. Cell (1998) [Pubmed]
  20. Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi. Wong, T.A., Fairn, G.D., Poon, P.P., Shmulevitz, M., McMaster, C.R., Singer, R.A., Johnston, G.C. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  21. Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity. Tang, X., Waksman, M., Ely, Y., Liscovitch, M. Eur. J. Biochem. (2002) [Pubmed]
  22. Molecular cloning of a gene encoding phospholipase D from the pathogenic and dimorphic fungus, Candida albicans. Kanoh, H., Nakashima, S., Zhao, Y., Sugiyama, Y., Kitajima, Y., Nozawa, Y. Biochim. Biophys. Acta (1998) [Pubmed]
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