Altered polyamine metabolism in the PRO/ Re strain of inbred mice.
The PRO/ Re strain of inbred mice are characterized by abnormally high concentrations of proline in both blood (hyperprolinaemia) and urine (prolinuria). They excrete increased amounts of polyamines in their urine. Male PRO/ Re mice excreted putrescine at 175% and spermidine at 300% the amount of male C57BL/6J controls. Female PRO/ Re mice excreted putrescine at 115% and spermidine at 150% of the amount in the urine of female controls. Examination of the enzymes involved in polyamine biosynthesis revealed that ornithine decarboxylase, the initial enzyme in the polyamine-biosynthetic pathway, was increased by 150% in the kidneys and by 100% in the liver of male PRO/ Re mice. There was no significant difference between PRO/ Re and C57BL/6J male mice for either putrescine- or spermidine- stimulated S-adenosylmethionine decarboxylase activity. Female PRO/ Re mice showed no significant difference from female C57BL/6J mice for any of the enzymes examined. When the concentrations of the polyamines in the tissues of the PRO/ Re mice were determined, spermidine and spermine concentrations in the kidneys of the male PRO/ Re mice were twice those of the controls. Spermidine concentration in the livers of both male and female PRO/ Re mice was approx. 130% that of the controls. Polyamine concentrations in the brains were similar in controls and mutants. The increased polyamine biosynthesis and excretion in the PRO/ Re mutant mice may be a mechanism to decrease the extent of proline accumulation.[1]References
- Altered polyamine metabolism in the PRO/Re strain of inbred mice. Manen, C.A., Blake, R.L., Russell, D.H. Biochem. J. (1976) [Pubmed]
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