Similarity in moth-fly specific larvicidal activity between two serologically unrelated Bacillus thuringiensis strains.
Parasporal inclusions of a Bacillus thuringiensis isolate designated 92-KU-105-9 ( H14/19) exhibited unusual larvicidal activity, specific for the moth-fly, Telmatoscopus albipunctatus (Diptera: Psychodidae), similar to that of a previously reported B. thuringiensis serovar leesis (H33) strain. The LC50 value of the purified inclusions was 4.92 micrograms ml-1 for the moth-fly larvae, while no mortality was shown in the mosquitoes Culex pipiens molestus and Anopheles stephensi, at protein concentrations up to 10 mg ml-1. Morphologically, the inclusion was a homogeneous globular body surrounded by an electron-dense, thick envelope. Multilamellar inner structure was evident between envelope membrane and inclusion matrix. SDS-PAGE revealed that the inclusions consist of five proteins with molecular masses of 72, 70, 68, 56 and 30 kDa. These proteins cross-reacted with the antibodies against inclusion proteins of the serovar leesis strain. High homologies existed in N-terminal amino acid sequences between the three major proteins (72, 70 and 68 kDa) and the two established protein classes, Cry4A and Cry10A.[1]References
- Similarity in moth-fly specific larvicidal activity between two serologically unrelated Bacillus thuringiensis strains. Higuchi, K., Saitoh, H., Mizuki, E., Ohba, M. FEMS Microbiol. Lett. (1998) [Pubmed]
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