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Chemical Compound Review:

SureCN87732 9H-fluorene-1,9-diamine

Synonyms: LS-69251, CTK8H1144, AC1MI9E5, 15824-95-0, Diaminofluorene, ...

Worthington, R.E. et al., Maack, C.A. et al., McGuckin, C.P. et al., Miwa, Y. et al.

McGuckin, Forraz, Liu,

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High impact information on Fluorenediamine

These red cells were stained well with 2,7-diaminofluorene (DAF), and therefore were erythroid cells [1].
Mutagenesis produced in mammary S9 activation of 2-AA, DAA or DAF was significantly inhibited by alpha-naphthoflavone (alpha NF) but was inhibited minimally by metyrapone (MP) [2].
Generation of fluorene blue (FB), the chromophore generated upon oxidation of DAF, was a linear function of Hb and erythrocyte concentration [3].
The DAF reagent was also used to stain both CFU-E and BFU-E colonies in situ, and was more sensitive than benzidine dihydrochloride in the histochemical detection of Hb [3].
Diaminofluorene stain detects erythroid differentiation in immature haemopoietic cells treated with EPO, IL-3, SCF, TGFbeta1, MIP-1alpha and IFNgamma [4].

References

Primitive erythropoiesis of mouse teratocarcinoma stem cells PCC3/A/1 in serum-free medium. Miwa, Y., Atsumi, T., Imai, N., Ikawa, Y. Development (1991) [Pubmed]
Procarcinogen activation by rat and human mammary extracts. Maack, C.A., Silva, M.H., Petrakis, N.L., Lee, R.E., Lyon, M. Carcinogenesis (1986) [Pubmed]
Quantitation of erythroid differentiation in vitro using a sensitive colorimetric assay for hemoglobin. Worthington, R.E., Bossie-Codreanu, J., Van Zant, G. Exp. Hematol. (1987) [Pubmed]
Diaminofluorene stain detects erythroid differentiation in immature haemopoietic cells treated with EPO, IL-3, SCF, TGFbeta1, MIP-1alpha and IFNgamma. McGuckin, C.P., Forraz, N., Liu, W.M. Eur. J. Haematol. (2003) [Pubmed]

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