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Gene Review:

tbpA - thiamine transporter substrate binding...

Escherichia coli CFT073

Gonzalez, G.C. et al., Ogunnariwo, J.A. et al., Loosmore, S.M. et al.

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Disease relevance of tbpA

The genomic transferrin receptor genes (tbpA and tbpB) from two strains of Haemophilus influenzae type b (Hib) and two strains of non-typable H. influenzae (NTHi) have been cloned and sequenced [1].
Several attempts at expressing the cloned tbpA gene were unsuccessful, suggesting that the product of the gene may be toxic to E. coli [2].
The tbpA and tbpB genes encoding the transferrin receptor proteins Tbp1 and Tbp2 from a serotype 7 strain of Actinobacillus pleuropneumoniae were cloned, sequenced, and expressed in Escherichia coli [3].
The tbpA and tbpB genes encoding the transferrin receptor proteins, TbpA and TbpB, from Pasteurella haemolytica A1 were cloned, sequenced and expressed in Escherichia coli [2].

High impact information on tbpA

The genes were organized in a putative operon arrangement of tbpB- tbpA [2].
Upon high-level expression of the tbpA gene, a large proportion of the recombinant Tbp1 was found in inclusion bodies and could not be affinity-isolated with immobilized porcine transferrin [3].

References

Cloning and expression of the Haemophilus influenzae transferrin receptor genes. Loosmore, S.M., Yang, Y.P., Coleman, D.C., Shortreed, J.M., England, D.M., Harkness, R.E., Chong, P.S., Klein, M.H. Mol. Microbiol. (1996) [Pubmed]
Characterization of the Pasteurella haemolytica transferrin receptor genes and the recombinant receptor proteins. Ogunnariwo, J.A., Woo, T.K., Lo, R.Y., Gonzalez, G.C., Schryvers, A.B. Microb. Pathog. (1997) [Pubmed]
Sequence, genetic analysis, and expression of Actinobacillus pleuropneumoniae transferrin receptor genes. Gonzalez, G.C., Yu, R.H., Rosteck, P.R., Schryvers, A.B. Microbiology (Reading, Engl.) (1995) [Pubmed]

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