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Gene Review:

ANP32B - acidic (leucine-rich) nuclear...

Homo sapiens

Synonyms: APRIL, Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, PHAPI2, SSP29, ...

Brennan, C.M. et al., Pegoraro, G. et al., Zhu, L. et al., Gallouzi, I.E. et al., Reilly, P., et al.

Mencinger, Panagopoulos, Contreras, Mitelman, Aman, Patrick Reilly,

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High impact information on ANP32B

The inhibition of CRM1 by leptomycin B leads to the nuclear retention of pp32 and APRIL, their increased association with HuR, and an increase in HuR's association with nuclear poly(A)+ RNA [1].
We show that pp32 and APRIL are nucleocytoplasmic shuttling proteins that interact with the nuclear export factor CRM1 (chromosomal region maintenance protein 1) [1].
All four ligands contain long, acidic COOH-terminal tails, while pp32 and APRIL share a second motif: rev-like leucine-rich repeats in their NH(2)-terminal regions [1].
we report that ANP32B expression is associated with a poor prognosis in human breast cancer [2]

Biological context of ANP32B

Its interaction with RNA polymerase I suggests that SSP29 may participate in signal transduction that directs nucleolar activities by regulating ribosomal RNA biosynthesis [3].

Anatomical context of ANP32B

SSP29 is expressed in all human tissues and cell lines tested, localized to nucleoplasm and translocated partially to the nucleoli after heat shock [3].

Physical interactions of ANP32B

Export after heat shock requires the same domains of HuR (HNS and RRM3) that are essential for binding pp32 and APRIL [4].

Co-localisations of ANP32B

Here we show that heat shock induces increased association of HuR with pp32 and APRIL through protein-protein interactions and that these ligands partially colocalize with HuR in cytoplasmic foci [4].

Other interactions of ANP32B

Further experiments revealed that silencing of ANP32A and ANP32B inhibited AAV replication, while overexpression of all of the components of the TAF-I/Set complex increased de novo AAV DNA synthesis in permissive cells [5].
Several polypeptides were identified by mass spectrometry, among which was ANP32B, a member of the acidic nuclear protein 32 family which takes part in the formation of the template-activating factor I/Set oncoprotein (TAF-I/Set) complex [5].

Analytical, diagnostic and therapeutic context of ANP32B

APRIL shows tissue-specific expression as shown by Northern blot analysis [6].

References

Protein ligands to HuR modulate its interaction with target mRNAs in vivo. Brennan, C.M., Gallouzi, I.E., Steitz, J.A. J. Cell Biol. (2000) [Pubmed]
Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development. Reilly, P.T., Afzal, S., Gorrini, C., Lui, K., Bukhman, Y.V., Wakeham, A., Haight, J., Ling, T.W., Cheung, C.C., Elia, A.J., Turner, P.V., Mak, T.W. Proc. Natl. Acad. Sci. U. S. A. (2011) [Pubmed]
Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR family. Zhu, L., Perlaky, L., Henning, D., Valdez, B.C. Biochem. Mol. Biol. Int. (1997) [Pubmed]
Protein ligands mediate the CRM1-dependent export of HuR in response to heat shock. Gallouzi, I.E., Brennan, C.M., Steitz, J.A. RNA (2001) [Pubmed]
Regulation of adeno-associated virus DNA replication by the cellular TAF-I/set complex. Pegoraro, G., Marcello, A., Myers, M.P., Giacca, M. J. Virol. (2006) [Pubmed]
Expression analysis and chromosomal mapping of a novel human gene, APRIL, encoding an acidic protein rich in leucines. Mencinger, M., Panagopoulos, I., Contreras, J.A., Mitelman, F., Aman, P. Biochim. Biophys. Acta (1998) [Pubmed]

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