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Gene Review

buk  -  butyrate kinase

Clostridium acetobutylicum ATCC 824

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Disease relevance of buk

  • The genes encoding both Clostridium acetobutylicum ATCC 824 butyrate synthesis pathway enzymes, phosphotransbutyrylase (ptb) and butyrate kinase (buk), were sequenced [1].
  • In this study, genes of butyrate kinase (buk) and phosphotransbutyrylase (ptb) from Clostridium acetobutylicum, and poly (3-hydroxybutyrate) (PHB) synthase gene from Thiocapsa pfennigii were used for construction of a metabolic pathway to synthesize the polythioesters [2].

High impact information on buk

  • DNA array-based transcriptional analysis of the buk and M5 mutants demonstrated that high BuP levels corresponded to downregulation of flagellar genes and upregulation of solvent formation and stress genes [3].
  • Here, we determined BuP and acetyl phosphate (AcP) levels in fermentations of C. acetobutylicum wild type (WT), degenerate strain M5, a butyrate kinase (buk) mutant, and a phosphotransacetylase (pta) mutant [3].
  • The predicted products of ptb and buk exhibited significant homology to the phosphotransbutyrylase and butyrate kinase, respectively, from Clostridium acetobutylicum [4].
  • The genes are immediately adjacent on the chromosome, with ptb preceding buk [1].
  • The ptb and buk genes appear to form an operon [1].

Biological context of buk

  • Non-replicative plasmid constructs, containing either clostridial phosphotransacetylase (pta) or butyrate kinase (buk) gene fragments, were integrated into homologous regions on the chromosome [5].

Associations of buk with chemical compounds

  • Inactivation of the buk gene reduced butyrate kinase activity, significantly decreased butyrate production and increased butanol production [5].


  1. Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824. Walter, K.A., Nair, R.V., Cary, J.W., Bennett, G.N., Papoutsakis, E.T. Gene (1993) [Pubmed]
  2. Biosynthesis of poly (3-mercaptopropionate) and poly (3-mercaptopropionate-co-3-hydroxybutyrate) with recombinant Escherichia coli. Liu, S.J., Lütke-Eversloh, T., Steinbüchel, A. Sheng Wu Gong Cheng Xue Bao (2003) [Pubmed]
  3. Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation. Zhao, Y., Tomas, C.A., Rudolph, F.B., Papoutsakis, E.T., Bennett, G.N. Appl. Environ. Microbiol. (2005) [Pubmed]
  4. Catabolism of branched-chain alpha-keto acids in Enterococcus faecalis: the bkd gene cluster, enzymes, and metabolic route. Ward, D.E., Ross, R.P., van der Weijden, C.C., Snoep, J.L., Claiborne, A. J. Bacteriol. (1999) [Pubmed]
  5. Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824. Green, E.M., Boynton, Z.L., Harris, L.M., Rudolph, F.B., Papoutsakis, E.T., Bennett, G.N. Microbiology (Reading, Engl.) (1996) [Pubmed]
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