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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

H3f3b  -  H3 histone, family 3B

Rattus norvegicus

Synonyms: H3.3b, Histone H3.3
 
 
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Analytical, diagnostic and therapeutic context of H3f3b

  • Using reverse transcriptase-coupled polymerase chain reaction (RT-PCR) and class-specific Ca2+ channel oligonucleotide probes, messenger RNA levels were measured and compared to Histone H3.3 transcript which remained relatively constant over the duration of NGF treatment [1].
  • Although more sensitive detection methods exist, such as the use of RNA probes or solution hybridization (RNase protection), we have found the most reliable, sensitive, rapid, and accurate method is the reverse transcription-polymerase chain reaction (RT-PCR) using histone H3.3 as an internal control for both steps of this procedure [2].

References

  1. Ca2+ channel alpha 1-subunit transcripts are differentially expressed in rat pheochromocytoma (PC12) cells following nerve growth factor treatment. Colston, J.T., Valdes, J.J., Chambers, J.P. Int. J. Dev. Neurosci. (1998) [Pubmed]
  2. Coupled reverse transcription-polymerase chain reaction (RT-PCR) technique is comparative, quantitative, and rapid: uses in alcohol research involving low abundance mRNA species such as hypothalamic LHRH and GRF. Kelley, M.R., Jurgens, J.K., Tentler, J., Emanuele, N.V., Blutt, S.E., Emanuele, M.A. Alcohol (1993) [Pubmed]
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