Disease relevance of tdh

• A family of ISVs were widely distributed in Vibrio species, often regardless of the possession of the tdh genes, and one to several copies of the ISVs per organism were detected [1].

High impact information on tdh

• At the amino acid level, TdhX was completely identical to TDH/I, although two base differences were found in the nucleotide sequences between tdhX and tdh/I [2].
• The density of total V. parahaemolyticus bacteria was determined using a probe binding to the tlh (thermolabile hemolysin) gene, and the density of pathogenic V. parahaemolyticus bacteria was determined by using a probe binding to the tdh (thermostable direct hemolysin) gene [3].
• The pandemic serovars were positive by group-specific PCR (GS-PCR) and a PCR specific for open reading frame ORF8 (ORF8-PCR), which are molecular markers of the pandemic clone, and were positive for tdh but negative for trh [4].
• While the virulence characteristics (tdh positive, trh negative) of the Spanish clinical isolates matched those of the O3:K6 clone from Asia and North America, they were clearly excluded from this clone by group-specific PCR, PFGE, and serotyping [5].
• Among these, 48 strains belonging to four serotypes had the pandemic genotype and possessed the tdh gene [6].

Chemical compound and disease context of tdh

• Detection of tdh and trh genes in a urea-hydrolysing environmental isolate of Vibrio parahaemolyticus from the Andamans [7].

Biological context of tdh

• The majority of strains (81%) had two copies of tdh on the chromosome, and no copies of trh or ure [8].

Other interactions of tdh

• The tdh gene of Vibrio parahaemolyticus which encodes the thermostable direct hemolysin has been found in some strains of other Vibrio species [1].

Analytical, diagnostic and therapeutic context of tdh

• The ribotypes of the strains of these serotypes were indistinguishable, except for a Japanese tdh- negative O3:K6 strain and a U.S. clinical O3:K6 isolate, which had slightly different profiles [9].
• Furthermore, the prevalence of V. parahaemolyticus was studied by PCR amplification of the toxR, tdh, and trh genes [3].
• Southern hybridization with a tdh probe, after pulsed-field gel electrophoresis of Notl-digested genomic DNA of each strain revealed only single bands, suggesting that the two copies of the exist on single Notl fragments in each strain [8].

References