Disease relevance of finP

• The genetic locations of traO, finP and tra-4 on the E. coli K12 sex factor F [1].

High impact information on finP

• These findings mean that the antisense finP RNA, thought to regulate traJ translation, must regulate traJ transcripts from both J and M promoters [2].
• The finP gene of plasmid R1 is located between the genes traM and traJ, partially overlapping the first few nucleotides of the latter [3].
• Repression and derepression of conjugation of plasmid R1 by wild-type and mutated finP antisense RNA [3].
• Mutagenesis of finP showed that only finP loop II mutants have lost the ability to repress conjugation of R1-19 in trans [3].
• Mutations interrupting potential open reading frames of finP do not abolish finP repressor activity [3].

Regulatory relationships of finP

• Hence finP acts as an antisense RNA blocking the expression of the traJ gene by interacting with traJ mRNA through loop II [3].

Other interactions of finP

• traJ sense RNA initiates at two different promoters in R100-1 and forms two stable hybrids with antisense finP RNA [2].

References