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Gene Review

traJ  -  TraJ protein

Escherichia coli

 
 
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Disease relevance of traJ

 

High impact information on traJ

  • Exchange of a serine residue (Ser-74), located in motif II, against alanine prevents formation of stable relaxosomes but strongly enhances topoisomerase activity of the combination TraI/TraJ on form I oriT DNA [4].
  • The relaxation nick site and a 19-base pair invert repeat sequence that is recognized by asymmetric binding of the RP4 TraJ protein are interspaced by 8 base pairs [5].
  • As indicated by traJ mutants, the TraJ protein is a constituent of the relaxosome, the initiation complex of transfer DNA replication [6].
  • TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence repetition within the transfer origin [6].
  • Transfer of plasmid RP4 during bacterial conjugation requires the plasmid-encoded TraJ protein, which binds to the transfer origin (Fürste, J. P., Pansegrau, W., Ziegelin, G., Kröger, M., and Lanka, E. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 1771-1775) [6].
 

Biological context of traJ

  • The FinOP system of F-like plasmids consists of an antisense RNA (FinP) and a 22 kDa protein (FinO) which act in concert to prevent the translation of TraJ, the positive regulator of the transfer operon [7].
  • The predicted amino acid sequence of the TraJ protein shows that this outer-membrane protein lacks a signal sequence [8].
  • The principal conclusion from our results is that the cpx mutations impair accumulation of the TraJ protein, thereby reducing tra gene expression [9].
  • We measured TraJ activity in vivo by expression of a traY'-'lacZ fusion gene and TraJ protein by immuno-overlay blot [9].
  • Our previous studies using TnphoA, signature-tagged mutagenesis and differential fluorescence induction identified several E. coli K1 determinants such as OmpA, Ibe proteins, AslA, TraJ and CNF1 contributing to invasion of HBMEC in vitro and traversal of the blood-brain barrier in vivo [10].
 

Associations of traJ with chemical compounds

 

Regulatory relationships of traJ

  • In contrast to the TraJ protein synthesized in large quantity by heat-induced lambda (traJ) lysogens, the TraJ protein synthesized in normal donor cells was soluble, even after sedimentation at 100,000 X g [1].
 

Other interactions of traJ

  • Alterations that reduced the effect of template supercoiling on apparent promoter strength in vitro also reduced the effect of the F plasmid TraJ protein on traY expression in vivo [11].

References

  1. Characterization of the F plasmid TraJ protein synthesized in F' and Hfr strains of Escherichia coli K-12. Cuozzo, M., Silverman, P.M. J. Biol. Chem. (1986) [Pubmed]
  2. Overproduction in Escherichia coli K-12 and purification of the TraJ protein encoded by the conjugative plasmid F. Cuozzo, M., Silverman, P.M., Minkley, E.G. J. Biol. Chem. (1984) [Pubmed]
  3. lambda-Transducing phages carrying transfer genes isolated from an abnormal prophage insertion into the traY gene of F. Johnson, D.A., Willetts, N.S. Plasmid (1983) [Pubmed]
  4. Concerted action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (TraI) of conjugative plasmid RP4. Pansegrau, W., Schröder, W., Lanka, E. J. Biol. Chem. (1994) [Pubmed]
  5. Covalent association of the traI gene product of plasmid RP4 with the 5'-terminal nucleotide at the relaxation nick site. Pansegrau, W., Ziegelin, G., Lanka, E. J. Biol. Chem. (1990) [Pubmed]
  6. TraJ protein of plasmid RP4 binds to a 19-base pair invert sequence repetition within the transfer origin. Ziegelin, G., Fürste, J.P., Lanka, E. J. Biol. Chem. (1989) [Pubmed]
  7. Differential levels of fertility inhibition among F-like plasmids are related to the cellular concentration of finO mRNA. van Biesen, T., Frost, L.S. Mol. Microbiol. (1992) [Pubmed]
  8. The control region of the F plasmid transfer operon: DNA sequence of the traJ and traY genes and characterisation of the traY leads to Z promoter. Fowler, T., Taylor, L., Thompson, R. Gene (1983) [Pubmed]
  9. Accumulation of the F plasmid TraJ protein in cpx mutants of Escherichia coli. Silverman, P.M., Tran, L., Harris, R., Gaudin, H.M. J. Bacteriol. (1993) [Pubmed]
  10. Current concepts on Escherichia coli K1 translocation of the blood-brain barrier. Xie, Y., Kim, K.J., Kim, K.S. FEMS Immunol. Med. Microbiol. (2004) [Pubmed]
  11. Contributions of promoter context and structure to regulated expression of the F plasmid traY promoter in Escherichia coli K-12. Gaudin, H.M., Silverman, P.M. Mol. Microbiol. (1993) [Pubmed]
 
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