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Gene Review

traM  -  transfer gene expression protein; similar...

Escherichia coli

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Disease relevance of traM


High impact information on traM

  • TraM is essential for F plasmid-mediated bacterial conjugation, where it binds to the plasmid DNA near the origin of transfer, and recognizes a component of the transmembrane DNA transfer complex, TraD [2].
  • Comparison of TraM to its Glu88 mutants predicted to stabilize the helical structure suggests that the protonated state is the active form for binding TraD in conjugation [2].
  • TraM of plasmid R1 controls transfer gene expression as an integrated control element in a complex regulatory network [3].
  • TraM is required for conjugation and is encoded on a separate operon between the origin of transfer and the traJ gene [4].
  • In light of TraM's regulatory functions in transfer gene expression, we propose that TraM could control conjugative DNA processing in response to intracellular levels of transfer proteins [5].

Biological context of traM

  • TraM of plasmid R1 regulates its own expression [6].
  • Here, we demonstrate that the host RNA binding protein, Hfq, represses TraJ and TraM synthesis by destabilizing their respective mRNAs [7].
  • Whether the interaction between TraM and TraD causes a transient or permanent anchoring of the F factor to the site of transfer is discussed [8].
  • One region, containing overlapping TraM and IHF binding sites, could be interpreted as containing two binding sites for each protein [9].

Analytical, diagnostic and therapeutic context of traM


  1. Crystallization and preliminary X-ray diffraction studies of the quorum-sensing regulator TraM from Agrobacterium tumefaciens. Vannini, A., Volpari, C., Di Marco, S. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
  2. Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM. Lu, J., Edwards, R.A., Wong, J.J., Manchak, J., Scott, P.G., Frost, L.S., Glover, J.N. EMBO J. (2006) [Pubmed]
  3. TraM of plasmid R1 controls transfer gene expression as an integrated control element in a complex regulatory network. Pölzleitner, E., Zechner, E.L., Renner, W., Fratte, R., Jauk, B., Högenauer, G., Koraimann, G. Mol. Microbiol. (1997) [Pubmed]
  4. Regulation of the expression of the traM gene of the F sex factor of Escherichia coli. Penfold, S.S., Simon, J., Frost, L.S. Mol. Microbiol. (1996) [Pubmed]
  5. Transfer protein TraM stimulates TraI-catalyzed cleavage of the transfer origin of plasmid R1 in vivo. Kupelwieser, G., Schwab, M., Högenauer, G., Koraimann, G., Zechner, E.L. J. Mol. Biol. (1998) [Pubmed]
  6. TraM of plasmid R1 regulates its own expression. Schwab, M., Reisenzein, H., Högenauer, G. Mol. Microbiol. (1993) [Pubmed]
  7. Hfq is a regulator of F-plasmid TraJ and TraM synthesis in Escherichia coli. Will, W.R., Frost, L.S. J. Bacteriol. (2006) [Pubmed]
  8. The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. Disqué-Kochem, C., Dreiseikelmann, B. J. Bacteriol. (1997) [Pubmed]
  9. Studies on the binding of integration host factor (IHF) and TraM to the origin of transfer of the IncFV plasmid pED208. Di Laurenzio, L., Scraba, D.G., Paranchych, W., Frost, L.S. Mol. Gen. Genet. (1995) [Pubmed]
  10. Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the substrate selector protein TraM. Beranek, A., Zettl, M., Lorenzoni, K., Schauer, A., Manhart, M., Koraimann, G. J. Bacteriol. (2004) [Pubmed]
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