Disease relevance of granulin

• Phylogenetic trees of the granulovirus genes egt, granulin, pk-1 and me53 were constructed using maximum parsimony and distance analyses [1].
• A very late baculovirus promoter motif, ATAAG, was found within the putative promoter region of the CfGV granulin gene [2].

High impact information on granulin

• The substitution was verified by Southern hybridization, and expression of granulin by the mutant virus, BmGran, was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by amino acid sequencing of the predominant protein of BmGran inclusion bodies (IBs) [3].
• The nucleotide sequence of the granulin gene of Choristoneura fumiferana granulovirus (CfGV) was determined [2].
• The granulin genes of Trichoplusia ni, Pieris brassicae and Cryptophlebia leucotreta granuloviruses showed homologies ranging from 76.7-80.5% for nucleotide sequences and 84.2-88.3% for amino acid sequences when compared to CfGV [2].
• Nevertheless, granulin was detected in the nucleus by immuno-gold staining, indicating that late gene expression occurred prior to nuclear membrane disruption [4].

Analytical, diagnostic and therapeutic context of granulin

• By using a specific PCR approach, however, chimerical fragments from the highly conserved granulin gene sequence could be observed in DNA preparations of virus progeny [5].
• Identification and sequence analyses of the granulin gene of Choristoneura fumiferana granulovirus [2].

References