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Gene Review:

N gene - nucleocapsid protein

Infectious hematopoietic necrosis virus

Gilmore, R.D. et al., Mourton, C. et al., Seo, J.Y. et al., Emmenegger, E.J. et al., Einer-Jensen, K. et al., et al.

Emmenegger, Meyers, Burton, Kurath,

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Disease relevance of N gene

Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus [1].
Interesting features of the IHNV nucleocapsid gene sequence elucidated by the sequencing of these clones include short homologies with N genes of other rhabdoviruses at the 5' and 3' nontranslated termini of the mRNA, as well as an exceptionally long 5' noncoding region of the mRNA, suggesting a leader RNA may be coupled to the N mRNA [2].
The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus [2].

High impact information on N gene

Four monoclonal antibodies directed against the viral nucleocapsid were produced; they all recognized the four viral hemorrhagic septicemia virus (VHSV) serotypes [3].
The complete nucleotide sequence of the infectious hematopoietic necrosis virus (IHNV) nucleocapsid gene has been determined using cDNA clones of genomic and messenger RNAs [2].
In contrast, fish injected with plasmids containing the N gene, G gene, or N + G gene mixture showed 70, 5, and 2.5% cumulative mortality, respectively [4].
RPA analyses, utilizing 4 probes, N5, N3 (N gene), GF (G gene), and NV (NV gene), determined that the haplotypes of all 3 genes demonstrated a consistent spatial pattern [5].

Biological context of N gene

However, the nucleotide sequence of an American isolate (Makah) revealed a unique 20 nucleotide sequence in close proximity to the N gene [6].

Associations of N gene with chemical compounds

Genes encoding the nucleocapsid protein (N) and the C-terminal half of the glycoprotein (G) were amplified by RT-PCR and separately cloned into the eukaryotic expression vector pcDNA 3 [4].

Analytical, diagnostic and therapeutic context of N gene

An antigen capture enzyme-linked immunosorbent assay (ELISA) based on the detection of the viral nucleocapsid (anti-N system) was developed for the diagnosis of viral hemorrhagic septicemia [3].

References

Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus. Bernard, J., Lecocq-Xhonneux, F., Rossius, M., Thiry, M.E., de Kinkelin, P. J. Gen. Virol. (1990) [Pubmed]
The nucleocapsid gene of infectious hematopoietic necrosis virus, a fish rhabdovirus. Gilmore, R.D., Leong, J.A. Virology (1988) [Pubmed]
Highly sensitive immunoassay for direct diagnosis of viral hemorrhagic septicemia which uses antinucleocapsid monoclonal antibodies. Mourton, C., Romestand, B., de Kinkelin, P., Jeffroy, J., Le Gouvello, R., Pau, B. J. Clin. Microbiol. (1992) [Pubmed]
Protection of flounder against hirame rhabdovirus (HIRRV) with a DNA vaccine containing the glycoprotein gene. Seo, J.Y., Kim, K.H., Kim, S.G., Oh, M.J., Nam, S.W., Kim, Y.T., Choi, T.J. Vaccine (2006) [Pubmed]
Genetic diversity and epidemiology of infectious hematopoietic necrosis virus in Alaska. Emmenegger, E.J., Meyers, T.R., Burton, T.O., Kurath, G. Dis. Aquat. Org. (2000) [Pubmed]
Use of the polymerase chain reaction (PCR) to differentiate serologically similar viral haemorrhagic septicaemia (VHS) virus isolates from Europe and America. Einer-Jensen, K., Olesen, N.J., Lorenzen, N., Jørgensen, P.E. Vet. Res. (1995) [Pubmed]

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