Disease relevance of env

• No amplification products were obtained from MNC DNA from the AI submissions using primer sets for both the BIV pol and env genes [1].

High impact information on env

• Numerous point mutations were also present; some caused coding substitutions that were most prevalent in the env encoding ORF [2].
• When compared to the nucleotide sequence of American BIV isolates, all Asian BIV field isolates seem to be smaller, several base substitutions were observed in the V1 region, and deletions were also found in the V2 region of env gene in these samples [3].
• PROCEDURE: Polymerase chain reaction testing that used 3 sets of primer pairs targeting pol and env regions of the BIV proviral genome was performed on DNA extracted from semen leukocytes, spermatozoa, and blood leukocytes from each bull [4].

Biological context of env

• Primers were designed to amplify 2 separate regions of the pol and env segments of the BIV genome [5].

Anatomical context of env

• Genomic DNA, derived from semen and buffy coat samples, was analyzed by nested polymerase chain reaction (PCR) using specific primers for the gag, pol, and env genes of BIV [6].

Analytical, diagnostic and therapeutic context of env

• Nested PCR also detected multiple-size env products in samples obtained later in the infection from the calf that received FL491, giving evidence that viral quasispecies were selected during in vivo replication of the virus [5].

References