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Gene Review

WSMVgp1  -  polyprotein

Wheat streak mosaic virus

 
 
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Disease relevance of WSMVgp1

  • Mapping of the P1 proteinase cleavage site in the polyprotein of Wheat streak mosaic virus (genus Tritimovirus) [1].
  • Monopartite members of the family Potyviridae utilize three virus-encoded proteinases to cleave the viral polyprotein into mature proteins [1].
  • An alignment of potyvirus polyprotein sequences in the carboxy-terminal region of the P1 domain revealed WSMV P1 contained conserved H(257), D(267), S(303) and FIVXG(325-329) residues upstream of the cleavage site that are typical of serine proteinases and shown by others to be required for P1 proteolysis in Tobacco etch virus [1].
 

High impact information on WSMVgp1

  • Inserts bearing the coding sequences of NPT II and beta-glucuronidase (GUS) were placed between the nuclear inclusion b (NIb) and coat protein (CP) domains of the wheat streak mosaic virus (WSMV) polyprotein ORF [2].
  • The WSMV NIb-CP junction containing the nuclear inclusion a (NIa) protease cleavage site was duplicated, permitting excision of foreign protein domains from the viral polyprotein [2].
  • Because the pathway of proteolytic maturation of the WSMV polyprotein has not been experimentally determined, random libraries of WSMV cDNA were made both in DNA-binding domain and activation domain plasmid vectors and introduced into yeast [3].
  • A comparison of this sequence along with the 3' terminal 1315 nucleotides of SCSMV-AP determined earlier with the other members of the family Potyviridae indicated that it had only 30% identity at the amino acid level for the partial polyprotein open reading frame (ORF) with the members of Ipomovirus and Tritimovirus genera [4].

References

  1. Mapping of the P1 proteinase cleavage site in the polyprotein of Wheat streak mosaic virus (genus Tritimovirus). Choi, I.R., Horken, K.M., Stenger, D.C., French, R. J. Gen. Virol. (2002) [Pubmed]
  2. A plant virus vector for systemic expression of foreign genes in cereals. Choi, I.R., Stenger, D.C., Morris, T.J., French, R. Plant J. (2000) [Pubmed]
  3. Multiple interactions among proteins encoded by the mite-transmitted wheat streak mosaic tritimovirus. Choi, I.R., Stenger, D.C., French, R. Virology (2000) [Pubmed]
  4. Taxonomic position of sugarcane streak mosaic virus in the family Potyviridae. Hema, M., Sreenivasulu, P., Savithri, H.S. Arch. Virol. (2002) [Pubmed]
 
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