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Gene Review:

Nat3 - N-acetyltransferase 3

Mus musculus

Synonyms: Aac3, Arylamide acetylase 3, Arylamine N-acetyltransferase 3, N-acetyltransferase type 3, NAT-3

Fretland, A.J. et al., Fakis, G. et al., Estrada-Rodgers, L. et al., Boukouvala, S. et al.

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High impact information on Nat3

In the present study, Nat1*, Nat2*8, and Nat3* were expressed in COS-1 cells, and their substrate selectivity was evaluated with various substrates [1].
For the substrates tested in this study, mouse Nat3 exhibited activity only toward 5-aminosalicylic acid and only at 1/20 the activity shown by Nat2 [1].
Recombinant NAT1, NAT2, and NAT3 proteins catalyzed N-, O-, and N,O-acetyltransferase activities [2].
NAT3 catalyzed aromatic amine N-acetyltransferase activities at very low rates, which confirms a previous study [2].
We show that Nat3 is close to Nat1 and Nat2, by screening of a P1 artificial chromosome (PAC) library and provide cytogenetic evidence for co-localisation of the three genes in chromosome region 8 B3.1-B3 [3].

Biological context of Nat3

The NAT1, NAT2, and NAT3 genes from C3H/HeJ and A/HeJ acetylator inbred mouse strains were amplified using the polymerase chain reaction, cloned into the plasmid vector pUC19, and sequenced [2].

Other interactions of Nat3

There are three functional genes in mice (Nat1, Nat2, and Nat3) encoding for three NAT isoenzymes [3].

Analytical, diagnostic and therapeutic context of Nat3

Activity of the NAT3 allozymes was hardly detectable, although the Nat3 gene does appear to be transcribed, since mRNA was detected by RT-PCR in the spleen [4].

References

Substrate selectivity of mouse N-acetyltransferases 1, 2, and 3 expressed in COS-1 cells. Estrada-Rodgers, L., Levy, G.N., Weber, W.W. Drug Metab. Dispos. (1998) [Pubmed]
Cloning, sequencing, and recombinant expression of NAT1, NAT2, and NAT3 derived from the C3H/HeJ (rapid) and A/HeJ (slow) acetylator inbred mouse: functional characterization of the activation and deactivation of aromatic amine carcinogens. Fretland, A.J., Doll, M.A., Gray, K., Feng, Y., Hein, D.W. Toxicol. Appl. Pharmacol. (1997) [Pubmed]
Chromosome mapping of the genes for murine arylamine N-acetyltransferases (NATs), enzymes involved in the metabolism of carcinogens: identification of a novel upstream noncoding exon for murine Nat2. Fakis, G., Boukouvala, S., Buckle, V., Payton, M., Denning, C., Sim, E. Cytogenet. Cell Genet. (2000) [Pubmed]
Identification and functional characterization of novel polymorphisms associated with the genes for arylamine N-acetyltransferases in mice. Boukouvala, S., Price, N., Sim, E. Pharmacogenetics (2002) [Pubmed]

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