High impact information on gly-13

• Expression in both insect cells and transgenic worms showed that gly-12 and gly-14, but not gly-13, encode active GnT I [1].
• We have cloned cDNAs for three predicted C. elegans genes homologous to mammalian GnT I (designated gly-12, gly-13, and gly-14) [1].
• However, lethality co-segregated with the gly-13 deletion within 0.02 map units (mu) in genetic mapping experiments, suggesting that the gly-13 mutation is responsible for the phenotype [2].
• Attempts to rescue the gly-13 null phenotype with the wild type transgene were not successful [2].
• Isolation of null alleles of the Caenorhabditis elegans gly-12, gly-13 and gly-14 genes, all of which encode UDP-GlcNAc: alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I activity [2].

Biological context of gly-13

• The gly-12 and gly-13 promoters were expressed from embryogenesis to adulthood in many tissues [1].
• We now describe a worm with null mutations in all three GnT I genes, gly-14 (III);gly-12 gly-13 (X) (III and X refer to the chromosome number) [3].

Other interactions of gly-13

• We report in this paper the preparation by ultra-violet (UV) light irradiation in the presence of trimethylpsoralen, of mutants lacking either gly-12, gly-13 or gly-14 [2].

References