High impact information on Hist1h2aa

• The synthesis of TH3 takes place in the spermatogonia but not in spermatocytes, in contrast to the other testis-specific histones, TH2A, H1t, and TH2B [1].
• Isolation and characterization of TH2A, a germ cell-specific variant of histone 2A in rat testis [2].
• TH2A gene is expressed only in testis [3].
• The deduced amino acid sequences show that H2A and TH2A histones have eight amino acid differences in the first half of the molecules and three consecutive changes in the C-terminal region [3].
• In addition, TH2A gene, like TH2B gene, contains the consensus sequence element in the 3' non-coding region which is involved in the S phase-specific stabilization of histone mRNA [3].

Biological context of Hist1h2aa

• Although synthesis of TH2A and TH2B histones is independent of DNA replication and insensitive to inhibitors of DNA synthesis in testis, the regulatory region shared by the two genes contain a bi-directional S phase-specific transcription regulatory element [3].
• While TH2A and TH2B are already present and actively synthesized in early primary spermatocytes (around the preleptotene stage), H1t does not accumulate until the pachytene stage [4].

Anatomical context of Hist1h2aa

• No detectable amounts of H1t, and at most, low levels of TH2A and TH2B could be found in spermatogonia [4].

References