High impact information on pim1

• We propose that pim1 is required for the reestablishment of nuclear structure following mitosis in fission yeast [1].
• A mutation in the RCC1-related protein pim1 results in nuclear envelope fragmentation in fission yeast [1].
• We show that the tomato Ran protein can suppress the Sch. pombe pim1 mutation, indicating that the tomato Ran protein and the Sch. pombe spi1 protein are functionally homologous [2].
• The distribution between the GTP- and GDP-bound forms of the protein is regulated by evolutionarily conserved gene products, rna1p and pim1p, functioning as GTPase-activating protein (GAP) and guanine nucleotide exchange factor (GEF), respectively [3].
• Localization of a nucleoplasmin-green fluorescent protein fusion suggests that ptr2 and ptr3 have defects also in nuclear protein import [4].

Associations of pim1 with chemical compounds

• We cloned the ptr2+ and ptr3+ genes and found that they encode Schizosaccharomyces pombe homologues of the mammalian RCC1, a guanine nucleotide exchange factor for RAN/TC4, and the ubiquitin-activating enzyme E1 involved in ubiquitin conjugation, respectively [4].

Other interactions of pim1

• Interaction of the pim1/spi1 mitotic checkpoint with a protein phosphatase [5].

Analytical, diagnostic and therapeutic context of pim1

• We isolated 16 temperature-sensitive (ts) mutants of S. pombeRCC1-homolog, pim1+, by error-prone PCR [6].

References