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Gene Review

SRM1  -  Srm1p

Saccharomyces cerevisiae S288c

Synonyms: Guanine nucleotide exchange factor SRM1, MTR1, PRP20, Pheromone response pathway component SRM1, Pre-mRNA-processing protein 20, ...
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Disease relevance of SRM1


High impact information on SRM1

  • We demonstrate that prp20 and rna1 mutants are very similar, if not identical, with respect to each of these biochemical phenotypes [2].
  • Previous work has demonstrated that prp20 mutants are defective in actin pre-mRNA splicing [2].
  • PRP20 is related, both in structure and function, to the RCC1 gene of mammals and the PIM1 gene of Schizosaccharomyces pombe, both of which appear to regulate entry into mitosis and chromosome condensation [2].
  • The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control [3].
  • Dhh1 is a cytoplasmic protein and is shown to be in a complex with the mRNA degradation factor Pat1/Mtr1 and with the 5'-3' exoribonuclease Xrn1 [4].

Biological context of SRM1


Associations of SRM1 with chemical compounds


Physical interactions of SRM1

  • Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue [8].

Regulatory relationships of SRM1

  • The predicted ATS1 protein contains 333 amino acids and has considerable structural homology to the products of both the mammalian mitotic control gene RCC1 and the S. cerevisiae gene SRM1/PRP20 [9].
  • Yap1p export is inhibited in both rna1-1 and prp20 (RanGNRF) mutant strains, but Yap1p rapidly accumulates at the nuclear periphery after shifting rna1-1, but not other mutant cells to the non-permissive temperature [10].
  • In yeast Rna1p is the GTPase activating protein for Ran (RanGAP) and Prp20p is the Ran GDP/GTP exchange factor (GEF) [11].

Other interactions of SRM1

  • Only CNR1 is essential, but both code for proteins extremely similar to Ran and can suppress mtr1 mutations in allele-specific fashion [12].
  • However, unlike Yrb1p, Yrb2p did not inhibit the nucleotide-releasing activity of Prp20p [13].
  • In vivo labeling experiments showed that Prp20p is a phosphoprotein; however, deletion of the KSP1 kinase did not affect Prp20p phosphorylation [5].
  • Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae [14].
  • The double mutant, srm1-1 ded1-21, could grow at 35 degrees C, but not at 37 degrees C. A revertant of srm1-1 ded1-21 that became able to grow at 37 degrees C acquired another mutation in the SRM1 gene, indicating the tight relationship between SRM1 and DED1 [15].

Analytical, diagnostic and therapeutic context of SRM1


  1. Prp20, the Saccharomyces cerevisiae homolog of the regulator of chromosome condensation, RCC1, interacts with double-stranded DNA through a multi-component complex containing GTP-binding proteins. Lee, A., Tam, R., Belhumeur, P., DiPaolo, T., Clark, M.W. J. Cell. Sci. (1993) [Pubmed]
  2. Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure. Forrester, W., Stutz, F., Rosbash, M., Wickens, M. Genes Dev. (1992) [Pubmed]
  3. The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control. Dilworth, D.J., Tackett, A.J., Rogers, R.S., Yi, E.C., Christmas, R.H., Smith, J.J., Siegel, A.F., Chait, B.T., Wozniak, R.W., Aitchison, J.D. J. Cell Biol. (2005) [Pubmed]
  4. The DEAD box protein Dhh1 stimulates the decapping enzyme Dcp1. Fischer, N., Weis, K. EMBO J. (2002) [Pubmed]
  5. Allele-specific suppression of a Saccharomyces cerevisiae prp20 mutation by overexpression of a nuclear serine/threonine protein kinase. Fleischmann, M., Stagljar, I., Aebi, M. Mol. Gen. Genet. (1996) [Pubmed]
  6. Yeast pheromone response pathway: characterization of a suppressor that restores mating to receptorless mutants. Clark, K.L., Sprague, G.F. Mol. Cell. Biol. (1989) [Pubmed]
  7. The yeast SRM1 protein and human RCC1 protein share analogous functions. Clark, K.L., Ohtsubo, M., Nishimoto, T., Goebl, M., Sprague, G.F. Cell Regul. (1991) [Pubmed]
  8. Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue. Taura, T., Schlenstedt, G., Silver, P.A. J. Biol. Chem. (1997) [Pubmed]
  9. Overexpression of yeast homologs of the mammalian checkpoint gene RCC1 suppresses the class of alpha-tubulin mutations that arrest with excess microtubules. Kirkpatrick, D., Solomon, F. Genetics (1994) [Pubmed]
  10. Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor. Yan, C., Lee, L.H., Davis, L.I. EMBO J. (1998) [Pubmed]
  11. Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution. Feng, W., Benko, A.L., Lee, J.H., Stanford, D.R., Hopper, A.K. J. Cell. Sci. (1999) [Pubmed]
  12. Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily. Kadowaki, T., Goldfarb, D., Spitz, L.M., Tartakoff, A.M., Ohno, M. EMBO J. (1993) [Pubmed]
  13. Yrb2p, a Nup2p-related yeast protein, has a functional overlap with Rna1p, a yeast Ran-GTPase-activating protein. Noguchi, E., Hayashi, N., Nakashima, N., Nishimoto, T. Mol. Cell. Biol. (1997) [Pubmed]
  14. Overexpression of Bud5p can suppress mutations in the Gsp1p guanine nucleotide exchange factor Prp20p in Saccharomyces cerevisiae. Clément, M., Lavallée, F., Barbès-Morin, G., de Repentigny, L., Belhumeur, P. Mol. Genet. Genomics (2001) [Pubmed]
  15. Genetic interaction of DED1 encoding a putative ATP-dependent RNA helicase with SRM1 encoding a mammalian RCC1 homolog in Saccharomyces cerevisiae. Hayashi, N., Seino, H., Irie, K., Watanabe, M., Clark, K.L., Matsumoto, K., Nishimoto, T. Mol. Gen. Genet. (1996) [Pubmed]
  16. Site-directed mutagenesis of the yeast PRP20/SRM1 gene reveals distinct activity domains in the protein product. Lee, A., Clark, K.L., Fleischmann, M., Aebi, M., Clark, M.W. Mol. Gen. Genet. (1994) [Pubmed]
  17. Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation. Fleischmann, M., Clark, M.W., Forrester, W., Wickens, M., Nishimoto, T., Aebi, M. Mol. Gen. Genet. (1991) [Pubmed]
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