High impact information on Fev

• Our results suggest that, during development of 5-HT neurons, Lmx1b is a critical intermediate factor that couples Nkx2-2-mediated early specification with Pet1-mediated terminal differentiation [1].
• Here we show that in mice lacking Pet-1, the majority of 5-HT neurons fail to differentiate [2].
• The Pet-1 ETS factor is a precise marker of developing and adult 5-HT neurons and is expressed shortly before 5-HT appears in the hindbrain [2].
• This approach is based on the ability of a Pet-1 enhancer region to direct reliable 5HT neuron-specific transgene expression in the CNS [3].
• Remarkably, Pet-1 RNA colocalizes with tryptophan hydroxylase-positive neurons in raphe nuclei but not with their nonserotonergic neuron or non-neuronal neighbors [4].

Biological context of Fev

• Lmx1b, Pet-1, and Nkx2.2 coordinately specify serotonergic neurotransmitter phenotype [5].
• These sites are capable of supporting transcriptional activation through interactions with the Pet-1 ETS domain and can function as enhancers [4].

Anatomical context of Fev

• The mPet-1 protein is clearly smaller since it lacks the first 103 N-terminal amino acids of rat Pet-1. mPet- 1 is expressed in central serotonergic (5-hydroxytryptaminergic) neurons located in the mes-/metencephalic raphe nuclei from E11 on until adulthood [6].

Associations of Fev with chemical compounds

• In these regions mPet-1 expression co-localizes precisely with the serotonin transporter (Sert),which it initially precedes [6].

Regulatory relationships of Fev

• Interestingly, mPet-1 was not found in neurons transiently expressing Sert [6].

Other interactions of Fev

• mPet-1, a mouse ETS-domain transcription factor, is expressed in central serotonergic neurons [6].

References