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Gene Review:

tfdS - ORF33

Ralstonia eutropha JMP134

This record was discontinued.

You, I.S. et al., Clément, P. et al., Matrubutham, U. et al., Laemmli, C.M. et al., Leveau, J.H. et al.

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Disease relevance of tfdS

Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134 [1].

High impact information on tfdS

Expression of tfdR and tfdS, the (identical) regulatory genes for the tfd pathway remained low and essentially unchanged during the entire adaptation phase [2].
In vivo recombinants of pYG1010 and a cloned tfdS gene rescued the 2,4-D phenotype, indicating that TfdS is a positive regulator of tfdA expression, but not for tfdCDEF expression [3].
Regulation of these operons by two unlinked genes, tfdR and tfdS, has been proposed [3].
The DNA sequence reveals 100% identify with the regulatory gene tfdS of pJP4 [1].

Biological context of tfdS

Physical and DNA sequence analyses revealed that the tfdR and tfdS genes were identical and were located within a longer inverted repeat of 1592 bp [3].
One reason why the tfd(II) genes do not disappear from plasmid pJP4 might be the necessity for keeping the regulatory genes for the 2,4-D pathway expression tfdR and tfdS [4].

Analytical, diagnostic and therapeutic context of tfdS

Sequence analysis of pJP4-F3 indicated that the plasmid had undergone a deletion of approximately 16 kb, which included the tfdA-tfdS intergenic region, spanning the tfdA gene to a previously unreported IS1071 element [5].

References

Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134. Matrubutham, U., Harker, A.R. J. Bacteriol. (1994) [Pubmed]
Dynamics of multigene expression during catabolic adaptation of Ralstonia eutropha JMP134 (pJP4) to the herbicide 2, 4-dichlorophenoxyacetate. Leveau, J.H., König, F., Füchslin, H., Werlen, C., Van Der Meer, J.R. Mol. Microbiol. (1999) [Pubmed]
Genetic and molecular analysis of a regulatory region of the herbicide 2,4-dichlorophenoxyacetate catabolic plasmid pJP4. You, I.S., Ghosal, D. Mol. Microbiol. (1995) [Pubmed]
Characterization of a second tfd gene cluster for chlorophenol and chlorocatechol metabolism on plasmid pJP4 in Ralstonia eutropha JMP134(pJP4). Laemmli, C.M., Leveau, J.H., Zehnder, A.J., van der Meer, J.R. J. Bacteriol. (2000) [Pubmed]
Molecular characterization of a deletion/duplication rearrangement in tfd genes from Ralstonia eutropha JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid. Clément, P., Pieper, D.H., González, B. Microbiology (Reading, Engl.) (2001) [Pubmed]

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