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Gene Review

tfdA  -  ORF34

Ralstonia eutropha JMP134

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Disease relevance of tfdA


High impact information on tfdA

  • A sharp increase in mRNA levels for tfdA, tfdCDEF-B, tfdDIICIIEIIFII-BII and tfdK was detected between 2 and 13 min after exposure to 2,4-D [4].
  • In vivo recombinants of pYG1010 and a cloned tfdS gene rescued the 2,4-D phenotype, indicating that TfdS is a positive regulator of tfdA expression, but not for tfdCDEF expression [5].
  • The plasmid genes specifying 2,4-D and 3-Cba catabolism are organized in three operons: tfdA, tfdB, and tfdCDEF [5].
  • The other recombinant plasmid (pJP4-FM) should harbor two copies of the tfdA gene but no copies of the tfd gene clusters [6].
  • Degradation of 4-chloro-2-methylphenoxyacetic acid in top- and subsoil is quantitatively linked to the class III tfdA gene [7].

Chemical compound and disease context of tfdA


Biological context of tfdA


Associations of tfdA with chemical compounds


Other interactions of tfdA

  • Even more so, one complete ISJP4 plus its tfdA-tfdS intergenic remnant were sufficient to mediate transposition of intervening DNA [13].

Analytical, diagnostic and therapeutic context of tfdA

  • Quantitative PCR revealed growth in the tfdA-containing bacterial community, from 500 genes g(-1) soil to approximately 3 x 10(4) genes g(-1) soil and to 7 x 10(5) genes g(-1) soil for topsoil initially added to 2.3 mg MCPA kg(-1) (dry weight) soil and 20 mg MCPA kg(-1) (dry weight) soil, respectively [7].


  1. Analysis, cloning, and high-level expression of 2,4-dichlorophenoxyacetate monooxygenase gene tfdA of Alcaligenes eutrophus JMP134. Streber, W.R., Timmis, K.N., Zenk, M.H. J. Bacteriol. (1987) [Pubmed]
  2. Purification and characterization of 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase. Fukumori, F., Hausinger, R.P. J. Biol. Chem. (1993) [Pubmed]
  3. 2,4-Dichlorophenoxyacetic acid-degrading bacteria contain mosaics of catabolic genes. Fulthorpe, R.R., McGowan, C., Maltseva, O.V., Holben, W.E., Tiedje, J.M. Appl. Environ. Microbiol. (1995) [Pubmed]
  4. Dynamics of multigene expression during catabolic adaptation of Ralstonia eutropha JMP134 (pJP4) to the herbicide 2, 4-dichlorophenoxyacetate. Leveau, J.H., König, F., Füchslin, H., Werlen, C., Van Der Meer, J.R. Mol. Microbiol. (1999) [Pubmed]
  5. Genetic and molecular analysis of a regulatory region of the herbicide 2,4-dichlorophenoxyacetate catabolic plasmid pJP4. You, I.S., Ghosal, D. Mol. Microbiol. (1995) [Pubmed]
  6. Molecular and Population Analyses of a Recombination Event in the Catabolic Plasmid pJP4. Larraín-Linton, J., De la Iglesia, R., Melo, F., González, B. J. Bacteriol. (2006) [Pubmed]
  7. Degradation of 4-chloro-2-methylphenoxyacetic acid in top- and subsoil is quantitatively linked to the class III tfdA gene. Baelum, J., Henriksen, T., Hansen, H.C., Jacobsen, C.S. Appl. Environ. Microbiol. (2006) [Pubmed]
  8. Duplication of a 2,4-dichlorophenoxyacetic acid monooxygenase gene in Alcaligenes eutrophus JMP134(pJP4). Perkins, E.J., Lurquin, P.F. J. Bacteriol. (1988) [Pubmed]
  9. Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC. Suwa, Y., Wright, A.D., Fukimori, F., Nummy, K.A., Hausinger, R.P., Holben, W.E., Forney, L.J. Appl. Environ. Microbiol. (1996) [Pubmed]
  10. Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134. Matrubutham, U., Harker, A.R. J. Bacteriol. (1994) [Pubmed]
  11. Utilization of phenoxyacetic acid, by strains using either the ortho or meta cleavage of catechol during phenol degradation, after conjugal transfer of tfdA, the gene encoding a 2,4-dichlorophenoxyacetic acid/2-oxoglutarate dioxygenase. Radnoti de Lipthay, J., Barkay, T., Vekova, J., Sørensen, S.J. Appl. Microbiol. Biotechnol. (1999) [Pubmed]
  12. Pristine environments harbor a new group of oligotrophic 2,4-dichlorophenoxyacetic acid-degrading bacteria. Kamagata, Y., Fulthorpe, R.R., Tamura, K., Takami, H., Forney, L.J., Tiedje, J.M. Appl. Environ. Microbiol. (1997) [Pubmed]
  13. Genetic characterization of insertion sequence ISJP4 on plasmid pJP4 from Ralstonia eutropha JMP134. Leveau, J.H., van der Meer, J.R. Gene (1997) [Pubmed]
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